https://www.selleckchem.com/products/tng260.html Statistical significant difference existed in the duration between ATC and DOS with DOT for ATT patient ( ≤ 0.05). Furthermore, a significant difference existed in the range of tongue movement between DOS and DOT in LTT patients ( ≤ 0.05). Remarkable changes in the tongue position were observed postinsertion of DOT in both anterior and LTT swallow patterns where the tongue tip and anterior tongue dorsum shifted upward toward the anterior palate resembling that of a mature swallow pattern. Remarkable changes in the tongue position were observed postinsertion of DOT in both anterior and LTT swallow patterns where the tongue tip and anterior tongue dorsum shifted upward toward the anterior palate resembling that of a mature swallow pattern. Tumor necrosis factor-alpha (TNF-α), a " major inflammatory cytokine," not only plays an important role in periodontal destruction but also is extremely toxic to the host. Till date, there are not many studies comparing the levels of TNF-α in serum and its relationship to periodontal disease. Our study aimed to compare the serum TNF-α among the two study groups, namely, healthy controls and chronic periodontitis patients and establish a correlation between serum TNF-α and various clinical parameters. Hence, an attempt is made to estimate the level of TNF-α in serum, its relationship to periodontal disease and to explore the possibility of using the level of TNF-α in serum as a biochemical " marker" of periodontal disease. Forty individuals participated in the study and were grouped into two subgroups. Group A - 20 systemically and periodontally healthy controls. Group B - twenty patients with generalized chronic periodontitis. The serum samples were assayed for TNF-α levels by enzyme-linked immunosorbensease. These observations suggest a positive association between periodontal disease and increased levels of TNF-α in serum. It can be concluded that there is a prospect of using the es