© 2020 Blackwell Verlag GmbH.INTRODUCTION We previously reported an antibody MIF-220 that recognizes a specific structure induced on the surface of thrombin-activated E-domain of one fibrin molecule bound with the D-domains of other fibrinogen/fibrin molecules. Utilizing MIF-220, we produced a test kit for cross-linked fibrin degradation products (XDP), LPIA-GENESIS D-dimer (LG-DD), and evaluated basic performance characteristics for clinical application. We then attempted to apply LG-DD to see its eligibility in clinical plasma samples. METHOD The characteristic performances requested for clinical use were studied including limit of quantitation, within-run imprecision, day-to-day imprecision, antigen excess, interference study, and method comparison with LPIAACE-Ddimer (ACE-DD) available on the market. RESULTS The performance characteristics were all satisfactory. Extraordinarily high concentrations of XDP are occasionally obtained by ACE-DD in samples with collection problems, but not by LG-DD, indicating that a certain XDP species present in the former was not measured by LG-DD. Structural studies suggested that the "B-b" set of polymerization sites must be involved as well in the maintenance of cross-linked fibrin in vivo. CONCLUSION LG-DD was able to measure a wide range of XDP, that is, 0.20-35.0 μg FEU/mL that covers the levels of XDP in most of the clinical samples. LG-DD was found to almost avoid false-positive results noticed in samples as mentioned above, and this feature seems to be preferable to established kits for the measurement of XDP. © 2020 LSI Medience Corporation. International Journal of Laboratory Hematology published by John Wiley & Sons Ltd.OBJECTIVE To examine the efficiency of hemoperfusion in removing South American rattlesnake (Crotalus durissus terrificus) venom from rats compared with neutralization by antivenom. DESIGN An exploratory experimental investigation in rats involving the injection of snake venom with or without subsequent hemoperfusion or antivenom administration. SETTING Basic animal research laboratory in a private university. ANIMALS Normal, healthy male Wistar rats (0.29-0.40 kg, 3-6 months old) from a commercial breeder. INTERVENTIONS Four experimental groups of randomly allocated rats (n = 3/group) were studied Group 1 rats were injected with a single dose of venom (5 mg/kg, IM, in the right thigh) with no other treatment; blood samples were collected minutes before death to determine leukocyte, platelet, and erythrocyte counts; Group 2 (Control) rats underwent hemoperfusion alone for 60 min using a hemoperfusion cartridge designed for protein adsorption (by granulated charcoal) and protein precipitation (by tannic acid);2020.BACKGROUND Capecitabine was previously used as a second-line or salvage therapy for metastatic nasopharyngeal carcinoma (NPC) and has shown satisfactory curative effect as maintenance therapy in other metastatic cancers. This study aimed to explore the role of capecitabine as maintenance therapy in de novo metastatic NPC patients with different plasma Epstein-Barr virus (EBV) DNA levels before treatment. METHODS We selected de novo metastatic NPC patients treated with locoregional radiotherapy (LRRT) for this retrospective study. The propensity score matching (PSM) was applied to balance potential confounders between patients who underwent capecitabine maintenance therapy and those who did not with a ratio of 13. Overall survival (OS) was the primary endpoint. The association between capecitabine maintenance therapy and survival was assessed using the log-rank test and a Cox proportional hazard model. RESULTS Among all patients eligible for this study, 64 received capecitabine maintenance therapy after LRRT. rsity Cancer Center.The photocatalytic reduction nitrogen (N2) to ammonia (NH3) has been considered as a promising strategy to alleviate human need and environmental pollution, for which developing photocatalyst is the effective method to complete the transformation of this process. We firstly design a series of highly efficient and stable polyoxometalates (POMs)-based zeolitic imidazolate framework-67 (ZIF-67) photocatalysts for N2 reduction. ZIF-67 can effectively fix N2 due to its porosity. Noted that the integration of POMs cluster contribute enormously advantages in terms of broadening absorption spectrum to improve the sunlight utilization, enhancing the stability of the materials, effectively inhibiting the recombination of photo-generated electron-hole pairs and reducing the charge transfer impedance. POMs can absorb light to convert into reduced POMs, which have stronger reducing ability to provide ample electrons to reduce N2. The reduced POMs can recover to the oxidation state by contacting with oxidant, which forms a self-recoverable and recyclable photocatalytic fixing N2 system. The photocatalytic activity enhances with the increasing number of substituted metals V in the POMs.  https://www.selleckchem.com/products/ptc-209.html  Satisfactorily, ZIF-67@K11[PMo4V8O40] (PMo4V8) displays the most significant photocatalytic N2 activity with the NH3 yield of 149.0 μmol L-1 h-1 improving by 83.5% (ZIF-67) and 78.9% (PMo4V8). The introduction of POMs provides new insights in design high-performance photocatalyst nanomaterials to reduce N2. © 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.A rapid, selective, and sensitive ultra-high performance liquid chromatography-tandem mass spectrometry method was developed for simultaneous determination of ferulic acid, paeoniflorin, and albiflorin, the major active constituents of Danggui-Shaoyao-San, in rat plasma using geniposide as the internal standard. The plasma samples were processed by protein precipitation with acetonitrile, and then separated on a Shim-Pack XR-ODS C18 column (75 mm × 3.0 mm, 2.2 μm) using gradient elution program with a mobile phase consisting of 0.1% aqueous formic acid and acetonitrile at a flow rate of 0.4 mL/min. The detection was achieved on a 3200 QTRAP mass spectrometer equipped with electrospray ionization source in negative ionization mode. Quantification was performed using multiple reaction monitoring mode by monitoring the fragmentation of m/z 192.9→134.0 for ferulic acid, m/z 525.0→120.9 for paeoniflorin, m/z 525.2→121.0 for albiflorin, and m/z 433.1→225.1 for the internal standard, respectively. The calibration curve was linear in the range of 5-2500 ng/mL for all the three analytes (r ≥ 0.