https://www.selleckchem.com/products/dasa-58.html , and secondary lactic acid fermenter Secundilactobacillus spp.. Operating at a higher OLR (20 gCOD L-1d-1) but HRT of 8.5 days, resulted in typical lactic acid fermentation (34 ± 5 gCOD L-1) harbouring a less diverse community (1D = 8.0 ± 0.7, 2D = 5.7 ± 0.9) rich in acid-resistant homofermentative Lactobacillus spp. These findings demonstrate that a flexible product portfolio can be achieved by small adjustments in two key operating conditions. This improves the economic potential of acidogenic fermentation for food waste valorisation.Substrate moisture content is an important but not well-understood variable in production and waste reduction processes that involves black soldier fly (BSF) larvae. The purpose of this paper is to characterise growth and metabolic performance of BSF larvae on substrate moisture contents from 45 to 85%. Larvae developed into prepupae only at 45-75% substrate moisture content. Within this interval, the maximal specific growth rate was highest (0.73 day-1), the growth period shortest (13 days), and the maximal dry weight lowest (88 mg) at 45% moisture content. Differences in cost of growth and maintenance were not observed at the different substrate moisture contents, and differences in larval performance were likely associated to differences in co-occurring microbial activities. As much as 22% of the substrate carbon was emitted as CO2 at 45% moisture content by microorganisms, measured as the difference between total respiration and larval respiration, whereas microbial CO2 production amounted to only 3% of the substrate carbon at 75% moisture content. As consequence of the high specific growth rate and short growth phase, the overall net growth efficiency was higher at 45% moisture content (0.62) than at 75% moisture content (0.52). Overall, the metabolic performance of the BSF larvae was insensitive to differences in substrate moisture content. Their performance was, however indirec