Background and objectives Non-steroidal anti-inflammatory drugs (NSAIDs) have been among the major causes of small intestinal injury in clinical practice. As such, the current study investigated the protective effect of 5-aminosalicylic acid (5-ASA) against an NSAID-induced small intestinal injury. Materials and Methods IEC-6 cells were treated with various concentrations of indomethacin with or without 5-ASA in a serum-free medium, after which an 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Dromide (MTT) assay, a cell apoptosis assay, a caspase-3 activity assay, a reactive oxygen species (ROS) content and Superoxide dismutase 2 (SOD2) activity measurement, a Western blotting for occludin and zonula occludens-1 (ZO-1) and a wound healing assay were conducted. Results 5-ASA ameliorated indomethacin-induced cell apoptosis and an increase in the intracellular ROS content while augmenting the indomethacin-induced suppression of SOD2 activity in IEC-6 cells. Moreover, 5-ASA reversed the indomethacin-induced attenuation of occludin and ZO-1 expression and promoted faster wound healing effects in IEC-6 cells following an indomethacin-induced injury. Conclusion Our results suggested that 5-ASA protects small intestinal cells against an NSAID-induced small intestinal injury by scavenging free radicals. Therefore, 5-ASA could be a potential treatment for an NSAID-induced small intestinal injury.Despite the updated knowledge of the impact of gut dysbiosis on diabetes, investigations into the beneficial effects of individual bacteria are still required. This study evaluates the antihyperglycemic efficacy of Lactobacillus paracasei HII01 and its possible mechanisms in diabetic rats. Diabetic rats were assigned to receive vehicle, L. paracasei HII01 (108 CFU/day), metformin 30 (mg/kg) or a combination of L. paracasei HII01 and metformin. Normal rats given vehicle and L. paracasei HII01 were included. Metabolic parameters, including in vitro hemi-diaphragm glucose uptake, skeletal insulin-signaling proteins, plasma lipopolysaccharide (LPS), gut permeability, composition of gut microbiota and its metabolites, as well as short-chain fatty acids (SCFAs), were assessed after 12 weeks of experiment. The results clearly demonstrated that L. paracasei HII01 improved glycemic parameters, glucose uptake, insulin-signaling proteins including pAktSer473, glucose transporter 4 (GLUT4) and phosphorylation of AMP-activated protein kinase (pAMPKThr172), tumor necrosis factor (TNF-α) and nuclear factor-κB (NF-kB) in diabetic rats. Modulation of gut microbiota was found together with improvement in leaky gut, endotoxemia and SCFAs in diabetic rats administered L. paracasei HII01. In conclusion, L. paracasei HII01 alleviated hyperglycemia in diabetic rats primarily by modulating gut microbiota along with lessening leaky gut, leading to improvement in endotoxemia and inflammation-disturbed insulin signaling, which was mediated partly by PI3K/Akt signaling and AMPK activation.There is a growing interest in the use of animal-assisted intervention (AAI) as a non-pharmacological strategy to improve child welfare in hospitals. However, the efficacy of implementation of programmes based on activities with dogs in a paediatric day hospital is not known. An intra-subject quasi-experimental longitudinal design was used for the purpose of assessing the feasibility of such an intervention, as well as exploring the perceptions of its outcomes by children and parents/guardians. A total of 55 children in outpatient care at a paediatric day hospital participated in an AAI session. The application of this intervention was feasible. Self-assessment by the paediatric patients revealed a statistically significant improvement in their emotional state after the session, which was large in size. Parents confirmed this improvement in their child, perceiving significant changes in their mood, which were large in size. This study suggests that AAI is an effective approach when it comes to promoting the emotional welfare of children during their stay in hospital care environments. Participating in recreational occupations with dogs could contribute in a relevant and efficient way to the development of significant and gratifying experiences and to a more positive perception of healthcare centres on the part of children.Over the past three decades, extensive research activity on Brillouin scattering-based distributed optical fiber sensors has led to the availability of commercial instruments capable of measuring the static temperature/strain distribution over kilometer distances and with high spatial resolution, with applications typically covering structural and environmental monitoring. At the same time, the interest in dynamic measurements has rapidly grown due to the relevant number of applications which could benefit from this technology, including structural analysis for defect identification, vibration detection, railway traffic monitoring, shock events detection, and so on. In this paper, we present an overview of the recent advances in Brillouin-based distributed optical fiber sensors for dynamic sensing. The aspects of the Brillouin scattering process relevant in distributed dynamic measurements are analyzed, and the different techniques are compared in terms of performance and hardware complexity.To date, little is known about influences on cows' physiological stress levels on farms. The present study explored associations of fecal cortisol metabolite concentrations (FCM) with (1) farm factors including human-animal contact, (2) cows' fear behaviors towards humans, and (3) milk production and udder health, involving 25 dairy farms and repeated fecal samples (n = 2625) from 674 focal cows. Farm factors via interviews and observations, avoidance distance (AD) and qualitative behavior assessment (QBA) during a human-animal interaction were recorded. Milk yield and somatic cell scores (SCS) were calculated from milk recordings. Levels of FCMs were in general relatively low. No associations with AD and milk yield could be detected. Correlations between FCMs and QBA and SCS were significant, but on a low level.  https://www.selleckchem.com/products/ver155008.html  Against expectations, FCMs were higher, when the farm provided concentrates by hand and habituated heifers to milking, in part possibly due to reversed cause-effect relations. Decreased FCM levels were found on farms that did not separate diseased cows, possibly due to the avoidance of social stress following changes in group structure.