The freeze-dried powder of Rubus coreanus Miquel ameliorated the isoproterenol-induced oxidative stress as well as tissue damage throughout rats.
 The liquid crystalline and rheological properties of chitin whiskers (CHWs) are significant for their application in fabrication of highly ordered composite materials and optical components. The aim of this work was to elucidate the influence of chemical structure and chargeability (zeta potential, electropositivity, electronegativity or zwitterionic character) on the liquid crystalline and rheological properties of CHWs. Firstly, CHWs with different chemical structure, including positively charged whiskers (CHWs and CHWs-D/60 min) and negatively charged whiskers (mCHWs), were designed via acid hydrolysis, deacetylation, and maleation, respectively. Subsequently, the chargeability of the above whiskers was further regulated by protonation or deprotonation. The whisker aqueous suspensions with high zeta potential behaved as nematic liquid crystals or chiral nematic liquid crystals, whereas those with low zeta potential had no liquid crystal characteristics. The viscosity, G', and G" values of the CHWs and CHWs-D/60 min aqueous suspensions treated with protonation were lower than those of the corresponding whiskers treated with deprotonation. However, the mCHWs exhibited different changes in their rheological properties under protonation or deprotonation due to the electronegativity and zwitterionic characteristics. In addition, the effects of ionic strength and pH on the liquid crystalline and rheological properties of CHWs, CHWs-D/60 min, and mCHWs aqueous suspensions varied since the chemical structure and chargeability of whiskers differ. V.Bovine viral diarrhea virus (BVDV) and bovine alphaherpesvirus type 1 (BoHV-1) are responsible for major economic losses of livestock worldwide, making their eradication an important objective of veterinary research. Vaccines against these infectious agents are commercially available but have some limitations due to the specific features of these viral agents. The development of new antiviral drugs is therefore essential. Native banana lectin (BanLec) is a lectin isolated from banana fruit (Musa acuminata) and has a high affinity for mannose glycans found in several viral envelopes. The inhibitory properties of this lectin against several viruses has already been demonstrated. The aim of this work was therefore to test the antiviral and virucidal activities of BanLec against BVDV-1 and BoHV-1. Its antiviral activity was assessed by measuring the viral titer and viability of susceptible Madin-Darby Bovine Kidney cells (MDBK) treated with BanLec before and after viral infection. The virucidal properties of BanLec were determined by preincubation of the lectin with the viruses, followed by measurement of the viral load in exposed cells. Treatment with 25 μg/mL BanLec resulted in high levels of inhibition against BVDV-1 (99.98%) and BoHV-1 (99.68%) without affecting cell viability, demonstrating promising potential as an antiviral agent. V.Gellan gum (GG), a nature-derived polysaccharide, is one of the materials widely used in cartilage tissue engineering (TE). Glycol chitosan (GC), a derivative of chitosan, is a water-soluble natural polymer that has excellent biocompatibility and biodegradability as well as cell adhesion. Herein, GG was physically blended with GC to enhance the mechanical properties and microenvironment of the GG to apply in cartilage TE. The study was conducted with a hydrogel model which is similar to the extracellular matrix (ECM) of cartilage tissue. The physicochemical studies were carried out with morphological study, swelling ratio, weight loss, and sol fraction. The mechanical characterization was conducted with compression test and rheological study to confirm availability in cartilage TE material. Furthermore, in vitro studies such as morphology investigation, viability assay, GAG content, qRT-PCR, and histological study were performed to verify biocompatibility and chondrogenesis of the material. The mechanical and biological properties improved with a proper amount of GC. Overall results verify the potential of the material and can be further used for the cartilage TE. V.Brigatinib, a multi-target kinase inhibitor, is primarily used to treat anaplastic lymphoma kinase (ALK)-positive patients with advanced non-small cell lung cancer (NSCLC) who have previously received crizotinib or are resistant to crizotinib. In this study, we focused on elucidating the interaction mechanism between brigatinib and human alpha-1-acid glycoprotein (HAG) through experimental and computational approaches.  https://www.selleckchem.com/products/ly333531.html  Steady-state fluorescence and UV-vis spectroscopy measurements revealed that brigatinib could quench the intrinsic fluorescence of HAG in a static quenching manner and formed the brigatinib-HAG complex with the stoichiometric ratio of 11. The findings revealed that brigatinib had a stronger affinity on HAG due to higher binding constant of 2.91 × 105 M-1 at 298 K.  https://www.selleckchem.com/products/ly333531.html  It can be proved from thermodynamic parameter analysis that brigatinib spontaneously bound to HAG in the means of enthalpy driven, the main forces for stabilizing brigatinib-HAG complexes were hydrogen bonding and hydrophobic interactions. The experimental results also indicated that the binding interaction induced micro-environmental changes around tryptophan residues and the alteration in secondary structure of HAG. The presence of metal ions like Mg2+, Zn2+, Ca2+, Ni2+ and Co2+ affects the binding interaction and thus change the therapeutic efficacy of brigatinib. Molecular docking results suggested that brigatinib was embedded to the hydrophobic cavity of HAG. The experimental and computational results certified that hydrogen bonding and hydrophobic interaction as well as electrostatic energy and van der Waals forces plays a leading role in the binding process. It has been suggested that a distributed oscillatory system in the brain operating in the theta (3.5-7 Hz) frequency range plays a major role in coordinating motor actions. The major objective of the present study was to explore the effects of human aging on the neurodynamics of motor-related EEG theta activity during correct motor response generation. Response-related potentials of young and older adults elicited in auditory and visual four-choice sensorimotor tasks were analyzed in the time-frequency domain. The phase-locking factor and total power were computed at motor cortical regions contra- and ipsilateral to the movement and at the midline to reflect phase synchronization and power of motor theta oscillations. Major results demonstrated that in both young and older adults, a pronounced response-locked theta activity (3.5-7 Hz) was generated at premotor, motor and sensorimotor cortical regions contra-lateral to the responding hand. Aging was associated with a decreased lateral asymmetry in the phase synchronization of only the left-hand responses.