The HA increased the electrostatic repulsion of all biochar colloids through adsorbed on the surface of biochar colloids, resulting in the increased steric hindrance and stability of biochar colloids, with the CCC increased from 75 to 624 mM to 827-1012 mM. Our findings reveal that soil kaolin, goethite, and humic acid colloids have remarkable effects on the stability and aggregation of biochar colloid, which will advance understanding of the potential environmental fate and behaviors of biochar colloids.Bacterial degradation is one of the most efficient ways to remove microcystins (MCs), the most frequently detected toxin in cyanobacterial blooms.  https://www.selleckchem.com/products/rvx-208.html  Using Novosphingobium sp. ERW19 as a representative strain, our laboratory previously demonstrated that quorum sensing (QS), the cell density-dependent gene regulation system, positively regulates biodegradation of MCs via transcriptional activation of mlr-pathway-associated genes. Increasing evidence indicates that QS is involved in a wide spectrum of regulatory circuits, but it remains unclear which physiological processes in MC degradation besides the expression of MC-degrading genes are also subject to QS-dependent regulation. This study used transcriptome analysis to identify QS-regulated genes during degradation of MCs. A large percentage (up to 32.6%) of the genome of the MC-degrading bacterial strain Novosphingobium sp. ERW19 was significantly differentially expressed in the corresponding QS mutants. Pathway enrichment analysis of QS-regulated genes revealed that QS mainly influenced metabolism-associated pathways, particularly those related to amino acid metabolism, carbohydrate metabolism, and biodegradation and metabolism of xenobiotics. In-depth functional interpretation of QS-regulated genes indicated a variety of pathways were potentially associated with bacterial degradation or physiological responses to MCs, including genes involved in cell motility, cytochrome P450-dependent metabolism of xenobiotics, glutathione S-transferase (GST), envelope stress response, and ribosomes. Furthermore, QS may be involved in regulating the initial and final steps of the catabolic pathway of phenylacetic acid, an intermediate product of MC degradation. Collectively, this global survey of QS-regulated genes in a MC-degrading bacterial strain facilitates a deeper understanding of QS-controlled processes that may be important for bacterial degradation of MCs or may contribute to the physiological responses of bacteria to MCs.Live fuel moisture content (LFMC), which is the ratio of water in the fresh biomass to the dry biomass, is a key variable that affects wildfire behaviour. Previous studies have assessed soil moisture as a predictor of LFMC over small areas with limited data, but a comprehensive evaluation at sub-continental scale is still lacking, and the explanatory utility has not been evaluated under different aridity conditions. In this study, the utility was evaluated using microwave soil moisture data from the ESA ECV_SM product from 1979 to 2018 and LFMC data from over 1000 sites in the coterminous United States. A time-lagged robust linear regression model was adopted, and the results were compared with analysis from in situ soil moisture measurements at adjacent sites. The results suggested that at most sites the LFMC correlates best with soil moisture within 60 days prior to LFMC sampling, and that the correlation is lower in areas with complex terrain. LFMC can be estimated from soil moisture with a mean RMSE of around 20%. The correlation between LFMC and soil moisture is significant (p less then 0.01) in most regions, and is mostly stable in different years. The major fuel types with a high response to soil moisture include pine, redcedar, sagebrush, oak, manzanita, chamise, mesquite and juniper, depending on the region. The LFMC ~ soil moisture correlation varies with the aridity condition, and soil moisture has a higher explanatory utility on LFMC under dry conditions. An analysis using SMAP Level-4 product indicated that the surface and root-zone soil moisture perform similarly in LFMC estimation. This study suggests that microwave soil moisture data contain sufficient information on LFMC, and may serve as a reference for the development of more sophisticated LFMC estimation methods.Managing phosphorus (P) is a global priority for environmental water quality due to P lost from agricultural land through leaching, runoff and subsurface flow. In Western Australia (WA), following decades of P fertiliser application to crops and pastures in low rainfall regions, questions have been raised about this region's contribution to environmental P loss. This study was conducted on the Fitzgerald River catchment in the south Western Australia (WA) with mixed cropping and grazing land uses and a Mediterranean climate with low mean rainfall (~350 mm yr-1). Phosphorus forms were monitored continuously over a three-year period in five separate streams, each draining a defined sub-catchment. The P concentrations in streams consistently exceeded Australian and New Zealand Environment Conservation Council (ANZECC) trigger values throughout the monitoring period. Of the measured total P concentration, ~75% was dissolved P (DRP; less then 0.45 μm) and 80% of that fraction was in the filterable reactive form (coarse textured sandy soils in higher rainfall areas of southwest WA. A reduction in DRP losses without yield loss could be achieved by following evidence-based fertiliser advice from soil testing to limit losses of legacy P".The lateral flux of dissolved organic carbon (DOC) from soils to inland waters and ultimately to the ocean represents a fundamental component of the global carbon cycle. To estimate the DOC flux, we developed an empirical terrestrial-aquatic DOC fluxes model (TAF-DOC). TAF-DOC incorporates various environmental factors (e.g., meteorology, sulfur, and nitrogen deposition) that to-date have not been comprehensively considered or well-represented in existing modeling frameworks. TAF-DOC was applied to estimate spatial-temporal patterns of DOC flux and potential fates across the conterminous United States during the 1985 to 2018 time period. Our results suggest that TAF-DOC successfully characterized spatial-temporal of DOC flux. As expected, the interannual pattern of DOC flux was strongly regulated by precipitation, but the long-term trend was significantly influenced by the rate of atmospheric wet sulfur deposition. From 1985 to 2018, TAF-DOC estimated DOC loading from terrestrial to aquatic ecosystems in the conterminous United States to be 33.