In this article, reference images for raters, and sample images used for rater training are provided. All raters independently indicated that the RGS score is significantly elevated with this methodology in reserpine-induced fibromyalgia-like rats.•The grimace scale, a method for rating facial expression, is applied to the reserpine-induced fibromyalgia-like rat, which manifests nociplastic pain.•Facial expression change in the reserpine-induced fibromyalgia-like rat is substantial and long-lasting.•Elevation of the RGS score in the reserpine-induced fibromyalgia-like rat may simulate spontaneous pain in patients with nociplastic pain. © 2020 The Author(s).The present protocol describes the synthesis of di and tri-substituted s-triazine derivatives•s-Triazine undergoes sequential nucleophilic substitution reaction but order of nucleophile is very crucial.•It is very difficult to substitute any nucleophile except amine once amine is incorporated onto s-triazine.•During the synthesis of O,N-type substituted s-triazine, always O-type should be incorporated first. © 2020 The Author(s). Published by Elsevier B.V.In this study, a new and effective catalyst for the synthesis of pyridazino[1,2-a]indazole, indazolo[2,1-b]phthalazine and pyrazolo[1,2-b]phthalazine derivatives was introduced. Triethanolammonium acetate ([TEAH][OAc]) accelerates the reaction in a one-pot and four-component condensation of aldehydes, hydrazine hydrate, succinic/phthalic anhydride, and 1,3-dicarbonyl compounds. The yield of the products is high, and the reaction conditions are mild and solvent-free. Furthermore, the model reaction was conducted in the presence of triethanolammonium sulphate ([TEAH][HSO4] and triethanolammonium formate ([TEAH][HCOO]) under various conditions. In addition, the catalyst is recyclable, therefore, it can be reused several times. The structure of the obtained products was confirmed by comparing the M.P., IR, and 1H NMR. Advantages of this technique are as following•Synthesis of novel, green, and one-pot and four-component condensation (4CC) under solvent-free conditions at room temperature.•The catalytic reaction is performed under mild and environmentally friendly conditions in short reaction times and excellent yields.•The catalyst is easily recycled and exhibits good chemical and structural stability. © 2020 The Author(s).Electrochemical energy devices, such as batteries and fuel cells, contain active electrode components that have highly porous, multiphase microstructures for improved performance. Predictive electrochemical models of solid oxide fuel cell (SOFC) electrode performance based on measured microstructures have been limited to small length scales, a small number of simulations, and/or relatively homogeneous microstructures. To overcome the difficulty in modeling electrochemical activity of inhomogeneous microstructures at considerable length scales, we have developed a high-throughput simulation application that operates on high-performance computing platforms. The open-source application, named Electrochemical Reactions in MIcrostructural NEtworks (ERMINE), is implemented within the MOOSE computational framework, and solves species transport coupled to both three-phase boundary and two-phase boundary electrochemical reactions.  https://www.selleckchem.com/products/bi-3802.html  As the core component, this application is further incorporated into a high-throughput computational workflow. The main advantages of the workflow include•Straightforward image-based volumetric meshing that conforms to complex, multi-phased microstructural features•Computation of local electrochemical fields in morphology-resolved microstructures at considerable length scales•Implementation on high performance computing platforms, leading to fast, high-throughput computations. © 2020 The Author(s). Published by Elsevier B.V.An animal model, suitable for resembling Parkinson's disease (PD) progress, should show both, motor and non-motor alterations. However, these features have been scarcely evaluated or developed in parkinsonian models induced by neurotoxins. This protocol provides modifications to original methods, allowing six different motor and non-motor behavior tests, which adequately and timely emulate the main parkinsonian sensorimotor alterations in the rat or mouse (1) bilateral sensorimotor alterations, examined by the vibrissae test; (2) balance and motor coordination, evaluated by the uncoordinated gait test; (3) locomotor asymmetry, analyzed by the cylinder test; (4) bradykinesia, as a locomotor alteration evidenced by the open field test; (5) depressive-like behavior, judged by the forced swimming test; and (6) hyposmia, assessed by the olfactory asymmetry test. Some advantages of using these behavioral tests over others include•No sophisticated materials or equipment are required for their application and evaluation.•They are used in rodent models for parkinsonian research, but they can also be helpful for studying other movement disorders.•These tests can accurately discriminate the affected side from the healthy one, after unilateral injury of one hemisphere, resulting in sensorimotor, olfactory or locomotor asymmetry. © 2020 The Authors. Published by Elsevier B.V.We present two new methods for simultaneous smoothing and sharpening of color images the GMS3 (Graph Method for Simultaneous Smoothing and Sharpening) and the NGMS3(Normalized Graph-Method for Simultaneous Smoothing and Sharpening). They are based on analyzing the structure of local graphs computed at every pixel using their respective neighbors. On the one hand, we define a kernel-based filter for smoothing each pixel with the pixels associated to nodes in its same connected component. On the other hand, we modify each pixel by increasing their differences with respect to the pixels in the other connected components of those local graphs. Our approach is shown to be competitive with respect to other state-of-the-art methods that simultaneously manage both processes.•We provide two methods that carry out the process of smoothing and sharpening simultaneously.•The methods are based on the analysis of the structure of a local graph defined from the differences in the RGB space among the pixels in a 3 × 3 window.