A unique feature of genodive is that it can also open data sets with nongenetic variables, for example environmental data or geographical coordinates that can be included in the analysis. In addition, genodive makes it possible to run several external programs (lfmm, structure, instruct and vegan) directly from its own user interface, avoiding the need for data reformatting and use of the command line. genodive is available for computers running Mac OS X 10.7 or higher and can be downloaded freely from http//www.patrickmeirmans.com/software. © 2020 The Authors. Molecular Ecology Resources published by John Wiley & Sons Ltd.BACKGROUND Although the utility of double-stranded RNA (dsRNA)-mediated knockdown as an environmentally friendly pest management strategy has gained traction in recent years, its overall efficacy has been limited by poor stability and limited cellular uptake. Encapsulation of dsRNAs with various nanomaterials, however, has shown promise in overcoming these limitations.  https://www.selleckchem.com/products/AdipoRon.html  This study sought to investigate the biological efficacy of an oral dsRNA nanomaterial mixture targeting the CYP15C1 gene product in the economically important rice pest, Chilo suppressalis. RESULTS A putative CYP15C1 ortholog was cloned from C. suppressalis midguts. The transcript is downregulated in fifth-instar larvae and is most highly expressed in heads. RNA interference (RNAi)-mediated knockdown of CsCYP15C1 was associated with significantly increased mortality. More importantly, feeding a dsRNA-nanomaterial mixture significantly increased larval mortality compared with feeding dsRNA alone. CONCLUSION A critical role for CsCYP15C1 function in molting is supported by sequence similarity with known juvenile hormone epoxidases, its expression profile, and abnormal molting phenotypes associated with RNA-mediated knockdown. CsCYP15C1 is thus a prime target for controlling C. suppressalis. Furthermore, RNAi-mediated characterization of candidate gene function can be enhanced by incorporating an enveloping nanomaterial. © 2020 Society of Chemical Industry. © 2020 Society of Chemical Industry.In the present work, a novel biocompatible scaffold was fabricated for the DNA aptamer immobilization. For the first time, amino-functionalized dendritic fibrous nanosilica (KCC-1-nPr-NH2 ) and gold nanoparticle supported by chitosan (AuNPs-CS) were synthesized and electrodeposited successfully on the surface of the glassy carbon electrode by chronoamperometry technique. Unique oligonucleotide of aflatoxin M1 (5'-ATC CGT CAC ACC TGC TCT GAC GCT GGG GTC GAC CCG GAG AAA TGC ATT CCC CTG TGG TGT TGG CTC CCG TAT) labeled by toluidine blue was immobilization on the prepared interface. Hence, a novel aptamer-based bioassay was formed for highly sensitive quantitation of AFM1 using cyclic voltammetry and differential plus voltammetry. The structure and morphology of GQDs-CS/KCC-1-nPr-NH2 were investigated by Fourier-transform infrared spectroscopy, X-ray diffraction, atomic force, scanning electron microscopy, and energy-dispersive X-ray spectroscopy. The achieved low limit of quantification of apta-assay for detection of AFM1 was 10fM. Also, calibration curve was linear from 0.1μM to 10fM in real samples. The proposed apta-assay has acceptable long-term stability. Designed aptasensor has a lot of remarkable advantages including excellent selectivity, sensitivity, and stability that could be used as facile bio-device for the determination of AFM1 in milk samples. © 2020 John Wiley & Sons Ltd.BACKGROUND MRI-based radiomics has been used to diagnose breast lesions; however, little research combining quantitative pharmacokinetic parameters of dynamic contrast-enhanced MRI (DCE-MRI) and diffusion kurtosis imaging (DKI) exists. PURPOSE To develop and validate a multimodal MRI-based radiomics model for the differential diagnosis of benign and malignant breast lesions and analyze the discriminative abilities of different MR sequences. STUDY TYPE Retrospective. POPULATION In all, 207 female patients with 207 histopathology-confirmed breast lesions (95 benign and 112 malignant) were included in the study. Then 159 patients were assigned to the training group, and 48 patients comprised the validation group. FIELD STRENGTH/SEQUENCE T2 -weighted (T2 W), T1 -weighted (T1 W), diffusion-weighted MR imaging (b-values = 0, 500, 800, and 2000 seconds/mm2 ) and quantitative DCE-MRI were performed on a 3.0T MR scanner. ASSESSMENT Radiomics features were extracted from T2 WI, T1 WI, DKI, apparent diffusion coefficienacokinetic parameter maps has a high discriminatory ability for benign and malignant breast lesions. LEVEL OF EVIDENCE 3 TECHNICAL EFFICACY STAGE 2. © 2020 International Society for Magnetic Resonance in Medicine.An epidermoid cyst is a common keratin-filled epithelial-lined cyst in clinic, which generally present as a slowly and progressively growing lesion. Surgical treatment is usually performed for epidermoid cysts. In this article, we describe a giant epidermoid cyst on the buttock, which has been growing for 20 years with no rupture. © 2020 Wiley Periodicals, Inc.WHAT IS KNOWN ABOUT THE SUBJECT? While therapeutic relationships remain core to mental health nursing practice and patient recovery, increased managerialism and focus on risk has impacted nurses' therapeutic practice with patients. While there is anecdotal evidence of the impact there has been little research that demonstrates nurses experience of therapeutic engagement within the current context. WHAT THE PAPER ADDS TO EXISTING KNOWLEDGE? The paper reports on qualitative research that highlights nurses' strong attempts to create the space for therapeutic engagement with clients. This research provides evidence of the constraints on practice imposed by new managerial processes and suggests potential means of responding to them. WHAT ARE THE IMPLICATIONS FOR PRACTICE? Mental health nurses are committed to working therapeutically but struggle to balance this against new managerial demands imposed across many OECD countries. The New Zealand government has recently reported on positive changes to mental health provision but does not suggest changes to the structures that impede good practice.