https://www.selleckchem.com/products/bromelain.html The existence of H2S in biogas may cause equipment corrosion and considerable SO2 emission. Commonly used biotrickling filters may cause biogas dilution or generation of explosive mixtures. Compared with biotrickling filters, two-step process such as bioscrubber filters can overcome these shortages. However, its removal efficiency was still limited due to low microbial activity under high pH condition. Here, a bioreactor filter was carried out under pH 9.0. Removal efficiency higher than 99% was achieved under sulfide loading rate reaching 4.24 kg S m-3d-1. Results of network and high throughput sequencing showed that Thiobacillus acted as both dominant species (accounting for 75%) and unique kinless hub in this bioreactor. Other bacteria (accounting for 25%) contributed 75% to the network, which implied the intensive interaction between Thiobacillus and others. Sulfide removal ability and pH tolerance of pure bacteria and mixed culture were considered to verify how microbial interaction influenced them. Compared with pure bacteria, mixed culture had better performance under high pH condition, which confirmed that microbial interaction promoted desulfurization efficiency under high pH condition. These results showed that intensive microbial interaction might be the key to enhance sulfide removal efficiency under high pH condition.Anionic Congo red dye (CR) is not effectively removed by conventional adsorbents. Three novel biochars derived from agro-waste (Acacia auriculiformis), modified with metal salts of FeCl3, AlCl3, and CaCl2 at 500 °C pyrolysis have been developed to enhance CR treatment. These biochars revealed significant differences in effluents compared to BC, which satisfied initial research expectations (P less then 0.05). The salt concentration of 2 M realized optimal biochars with the highest CR removal of 96.8%, for AlCl3-biochar and FeCl3-biochar and 70.8% for CaCl2-biochar. The modified biochars we