https://www.selleckchem.com/products/6-benzylaminopurine.html OBJECTIVES To report the draft genome sequences of two multidrug-resistant bacteria,Bacteroides thetaiotaomicron F9-2 and Escherichia coli 09-02E, isolated from stool samples of a healthy resident in Vietnam. METHODS Genome sequences were determined using the MiSeq and MinION platforms. Genomic assembly was performed using Platanus and Canu. The DDBJ Fast Annotation and Submission Tool were used for genome annotation. RESULTS The genome ofB. thetaiotaomicron F9-2 comprised 6,283,774 bp with a 42.7% GC content and 4,802 protein-coding sequences, whereas the E. coli 09-02E genome comprised 5,246,320 bp with a 50.6% GC content and 4,991 protein-coding sequences. Both strains harbored common antibiotic resistance genes, such as those for sulfonamide (sul2) and aminoglycoside (strA and strB). However, the sul2-strA-strB cassette was located on the chromosome and plasmid of strains F9-2 and 09-02E, respectively. These genes were flanked by different insertion sequences. CONCLUSIONS Considering their diversities in the human gut resistome, these strains would be of considerable interest for detailed comparative genomic analysis. Notably, the samesul2 cassette was found in facultative and obligate anaerobic bacterial isolates (resident in humans). However, the differential location of the cassette indicates a possible mechanism of gene transfer among gut microbes. OBJECTIVES Recently, mecA-negative Staphylococcus aureus strains with decreased susceptibility to oxacillin have been sporadically reported worldwide. They have been called borderline oxacillin-resistant S. aureus (BORSA). S. aureus β-lactamase (BlaZ), which is encoded by the blaZ gene, is categorized as a class A β-lactamase (penicillinase); hence, its hydrolytic activity against oxacillin, cephems, and carbapenems is low. The aim of this study was to clarify the mechanism of oxacillin resistance in BORSA. METHODS Clinical BORSA (MIC = 1 μg/ml) and met