https://www.selleckchem.com/btk.html Online learning (OLL) methodology has been incorporated in higher education extensively on the mount over the last few decades and with the onset of COVID-19 situation, the virtual method in academia became essential. After observing worldly destructions and death due to coronavirus, the WHO declared a high alert emergency, and since Pakistan started to follow lockdown since March 2020 to prevent high penetration and consequently, the online teaching method was adapted to keep the learning atmosphere alive. The study is aimed to identify the influencing factors and compliance for the implementation of the OLL system in COVID-19 and, in the future, nonpandemic state as well. The present study is designed to observe the adaptability and hurdles among pharmacy students, and for the purpose of the study, a questionnaire on the Likert scale was developed and asked to respond from pharmacy undergraduate and postgraduate students after taking consensus, which further analyzed through IBM SPSS Statistics Version f with an advanced characteristic. Online self-assessment tools have become an important asset among current teaching, learning, and assessment methods, especially among medical students. Developments in information technology and recent changes in methods of undergraduate learning, especially with the current COVID-19 pandemic situation, will provide an impetus among students to uptake this assessment tool. The study is aimed at bringing out the perception of medical students in using an online self-assessment tool in Biochemistry on the topics "Liver Function Tests" and "Renal Function Tests." This study was an observational study conducted among 150 1 - year medical students employing an online self-assessment tool in Biochemistry, and the perception response toward the questionnaire was graded. The participation was 100%, with the perception questionnaire having a good internal validity (α = 0.847). The students' response was