https://www.selleckchem.com/products/mcc950-sodium-salt.html Arterial blood samples were collected prior to, 5 and 30 minutes after puncture of pleura parietalis. In 10 horses, resection of the fifth rib was adequate for exposure of the heart. In one horse, removal of the sixth rib was also necessary. The duration of the surgical procedure (thoracotomy, pericardiotomy) was less then 45 minutes. During a thoracotomy, PaO2 decreased significantly (P less then .05) from 291.8 ± 82.8 mmHg to 165.2 ± 73.5 mmHg but was not different from normal anesthetized controls. The PaCO2 remained within normal limits. This surgical approach provided access to the entire circumference of the heart.Raman spectroscopy is a rapid qualitative and quantitative technique that allows the simultaneous determination of several components, both biomolecules both chemical compounds, in the biological fluids to assess the metabolic status. In this study, the serum composition was evaluated in regularly trained athletic horses using Raman spectroscopy to identify biomarkers of sports performance. Five clinically healthy and regularly trained Italian Saddle horses were subjected to a standardized obstacle course (350 m/minute; eleven 1.25 high jumps) preceded by a warm-up. On the collected sera, at rest, immediately after exercise, 30 minutes, and 1 hour after the end of the exercise Raman measurements were performed using a diode laser with the excitation wavelength of 785 nm. The analysis of the obtained spectra allowed the identification of peaks and bands different in position and intensity among the experimental conditions. The acquired spectra, obtained from horse sera collected during the experimental protocol, were visually similar, except for the large band detected in the 1,250-1,800 cm-1 range. The spectral intensity of the Raman spectrum decreased after training and 30 minutes after the end of exercise respect to the before exercise value, to come to the basal value after 60 minutes th