In this region, a combination of transcriptome analysis with gene annotations revealed three candidate genes SIN_1004875, SIN_1004882, and SIN_1004883 associated with leaf growth and development in sesame. These findings provided insight into the genetic characteristics and variability for sesame leaf and set up the foundation for future genomic studies on sesame leaves and will serve as gene resources for improvement of sesame plant architecture.Objective To establish a high performance liquid chromatography tandem mass spectrometry (HPLC / MS) method for the simultaneous determination of three antidepressant drugs in feces. Methods Samples were pretreated with n-hexane isopropanol (955, v/v). Gradient elution was carried out with mixed liquid of ultrapure water and acetonitrile as mobile phase and separated by Agilent ZORBAX SB-C18 liquid chromatography column (2.1 mm×100 mm, 3.5 m). The samples were detected by electrospray ionization tandem mass spectrometry and quantified by internal standard method. Results The recoveries of duloxetine, fluoxetine and escitalopram in fecal samples were 61.6% - 116.5%, with precision of 2.80% - 12.9% (n=5). The correlation coefficients (r) of linear equations were all greater than 0.995. The detection limits were 0.1, 1, and 0.001 μg/g, and the limits of quantification were 0.5, 2 and 0.005 μg/g, respectively. Conclusion The method is simple and accurate to detect the contents of three antidepressants in feces, such as duloxetine, fluoxetine and escitalopram.Objective To investigate the effect of nanoscale zirconium-porphyrin metal-organic framework (NPMOF) on the development of nervous system in larval zebrafish. Methods Embryos of zebrafish were incubated to E3 medium (n=500) or 100 mg/L NPMOF-E3 medium (n=500) from 6 hours post fertilization (hpf) to 28, 48, 72, 96 or 120 hpf. At 28, 48, 72, 96 and 120 hpf, 60 fish were collected respectively for quantitative real-time PCR in both groups. At 120 hpf, 20 fish were used for in situ hybridization, 150 fish were used for immunofluorescence and 30 fish were used for behavioral test, respectively. The shape and size of NPMOF was measured by TEM, and the optical properties were detected by UV-Vis and Fluorescence Spectrometer. In vivo development of multiple neurocytes was examined via in situ hybridization, immunofluorescence and quantitative real-time PCR. Behavioral test was used to manifest the locomotor changes of larval zebrafish. Results Compared to control group, the mRNA expression levels of neurodevelopment-relative, neuron-relative and neuroglia-relative genes were partially increased obviously after NPMOF exposure (P＜ 0.05). The distribution and phenotype of neurons and oligodendrocytes showed no significant differences between exposed and unexposed groups, while exposed group showed an increase in the number of müller glia and astrocytes (P＜0.05). In behavioral test, there was an increase in total movement distance, fast movement time and velocity and a decrease in total rest time following NPMOF exposure (P＜0.05). Conclusion The data indicate the potential facilitating effect of 100 mg/L NPMOF on neurodevelopment in vivo, especially on the growth of müller glia and astrocytes.Objective To investigate the protective effect and immune mechanism of berberine on cerebral ischemia/reperfusion injury in rats. Methods Fifty SD rats were randomly divided into sham operation group (Sham), model group (Model), berberine low dose groups (BBR-L, 25 mg/kg), berberine medium dose groups (BBR-M, 50 mg/kg) and berberine high dose groups (BBR-H, 100 mg/kg), with 10 rats in each group. Longa suture method was used to establish a rat model of cerebral ischemia/reperfusion, after 2 hours of ischemia, reperfusion for 24 hours. Rats in BBR-L, BBR-M and BBR-H were treated with berbrerine by gavage 2 hours after successful model building, while the sham operation group and the modle group were given the same volume of saline as described above. After 24 hours of administration, the activity of antioxidant enzymes superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), cytokine tumor necrosis factor α (TNF-α) , interferon β (IFN-β) , interleukin 6 (IL-6) and nitric oxide (NO) content were detected increased (P＜0.05), while the activities of SOD, GSH-Px and the levels of CD4+, CD8+ and CD4+/CD8+ in serum were decreased (P＜0.05). Conclusion Berberine may reduce oxidative stress, inhibit inflammation, enhance immune function, and reduce cerebral ischemia/reperfusion injury in rats, which may be related to the inhibition of NF-κB-NLRP3 signaling.Objective To investigate the effect of thyroxine (T4) on the expression of hypoxia inducible factor-1α (HIF-1α) in rat brain after aneurysmal subarachnoid hemorrhage (SAH) and its mechanism. Methods Seventy-two adult male SD rats were randomly divided into the following 4 groups subarachnoid hemorrhage model group(SAH), subarachnoid hemorrhage model and T4 group (SAH with T4), subarachnoid hemorrhage model with normal saline group (SAH with vehicle), and sham-operation group, 18 rats in each group. The model of subarachnoid hemorrhage group was established by internal carotid artery puncture.  https://www.selleckchem.com/products/nivolumab.html  CT plain scan was performed after the modeling immediately, T4 was administrated by intraabdominal injection of 3 μg/100 g every 24 hours for 3 days. SAH with T4 group was treated with thyroxine. SAH with vehicle group was treated with equal volume vehicle, all of them were killed 72 hours after modeling. The brain water content was determined to evaluate the brain edema, the apoptosis of cerebral cortex cells was detectcreased, the rat behavior was improved and the brain was protected.Objective To investigate the effects and molecular mechanisms of Second mitochondria-derived activator of caspase N7 (SmacN7) on the apoptosis of breast cancer cells MDA-MB-157. Methods Breast cancer cells MDA-MB-157 were treated with SmacN7 at the concentrations of 0-20 μmol/L. The proliferation activity of the cells was detected by MTS method, apoptosis and cell cycle were analyzed by flow cytometry, karyotypic changes of MDA-MB-157 cells were observed by Hoechst33342 staining, mitochondrial membrane potential was detected by JC-1 staining, and LDH release experiment was used to detect the drug cytotoxicity. Real time PCR was used to analyze the transcription levels of genes in MDA-MB-157 cells. The effect of inhibiting breast cancer proliferation was confirmed by tumor inhibition experiments. Results After treated with SmacN7, the inhibition rate of proliferation and apoptosis rate of breast cancer cells MDA-MB-157 were increased (P＜0.01), the karyotype changed significantly, the mitochondrial membrane potential in cells was decreased, and the LDH release was increased.