000).
 
  Our results demonstrated for the first time that miR-3650 was markedly upregulated in NPC tissues and positively associated with tumor progression and poor survival, suggesting that miR-3650 may be a potential novel prognostic biomarker and therapeutic target for NPC patients.
 Our results demonstrated for the first time that miR-3650 was markedly upregulated in NPC tissues and positively associated with tumor progression and poor survival, suggesting that miR-3650 may be a potential novel prognostic biomarker and therapeutic target for NPC patients.Phyllodes tumors of the breast are rare fibroepithelial tumors, and malignant phyllodes tumors occasionally exhibit heterogeneous differentiation. Here, we report a case of malignant phyllodes tumor with pleomorphic liposarcomatous differentiation, and examined the genomic features of both components using capture-based next-generation sequencing of 425 cancer-related genes. A 56-year-old woman presented with a palpable, asymptomatic nodule in the right breast measuring 4.0 cm × 3.0 cm. Based on the findings from microscopic examination, the lesions were identified as malignant phyllodes tumor and pleomorphic liposarcoma. The liposarcomatous differentiation component was positive for S-100, but had no MDM2 and CDK4 amplifications according to the results of fluorescence in situ hybridization. The malignant phyllodes tumor and liposarcomatous differentiation component had similar genetic mutations, such as TP53, TERT, EGFR, RARA, RB1, and MED12 mutations, all of which are common mutations in phyllodes tumors. These results indicate that the pathogenesis of the pleomorphic liposarcomatous differentiation was similar with that of phyllodes tumor, but was different from the pleomorphic liposarcoma in extramammary sites. Thus, the intratumoral heterogeneity may have independently evolved after tumorigenesis.
  Accumulating studies have demonstrated that long noncoding RNA plays a vital role in cancer progression. A previous study reported that LINC00665 was overexpressed and acted as a key tumor promoter in lung cancer, but the role of LINC00665 in gastric cancer (GC) remained uncharacterized. Thus, this study aimed to explore the mechanism of LINC00665 in GC.
 
  LINC00665 expression was explored using the Cancer Genome Atlas (TCGA), and a meta-analysis was conducted to assess the expression and prognostic value of LINC00665 in GC from Gene Expression Omnibus databases and the TCGA dataset. Real-time polymerase chain reaction (RT-PCR) was then conducted to verify the LINC00665 expression in GC tissues and cell lines. The effects of LINC00665 on cell proliferation, invasion, metastasis, and cell cycle in GC were evaluated using the CCK-8, wound healing, Transwell, and flow cytometry assays. In vitro validation was also performed.
 
  LINC00665 overexpression was found in GC, and LINC00665 upregulation was significantly related to poor overall survival and disease-free survival. LINC00665 expression was associated with tumor depth, lymph node metastasis, and TNM stage. Univariate and multivariate analyses proved that LINC00665 could be an independent prognostic biomarker in GC. LINC00665 knockdown subsequently inhibited cell proliferation, invasion, and metastasis in GC cell lines; promoted cell apoptosis; and arrested GC cell lines in the G0/G1 phase. Western blot analysis indicated that LINC00665 silencing inhibited epithelial-mesenchymal transition and decreased the expression levels of TGF-β1, Smad2, and α-SMA.
 
  LINC00665 can be a potential diagnostic and prognostic biomarker for GC patients, and LINC00665 promotes GC cell proliferation, invasion, and metastasis by activating the TGF-β signal pathway.
 LINC00665 can be a potential diagnostic and prognostic biomarker for GC patients, and LINC00665 promotes GC cell proliferation, invasion, and metastasis by activating the TGF-β signal pathway.East Asian oceans are possibly affected by a high nitrogen (N) burden because of the intense anthropogenic emissions in this region. Based on high-resolution regional chemical transport modeling with horizontal grid scales of 36 and 12 km, we investigated the N burden into East Asian oceans via atmospheric deposition in 2010. We found a high N burden of 2-9 kg N ha-1 yr-1 over the Yellow Sea, East China Sea (ECS), and Sea of Japan. Emissions over East Asia were dominated by ammonia (NH3) over land and nitrogen oxides (NOx) over oceans, and N deposition was dominated by reduced N over most land and open ocean, whereas it was dominated by oxidized N over marginal seas and desert areas.  https://www.selleckchem.com/products/4sc-202.html  The verified numerical modeling identified that the following processes were quantitatively important over East Asian oceans the dry deposition of nitric acid (HNO3), NH3, and coarse-mode (aerodynamic diameter greater than 2.5 μm) NO3-, and wet deposition of fine-mode (aerodynamic diameter less than 2.5 μm) NO3- and NH4+. The relative importance of the dry deposition of coarse-mode NO3- was higher over open ocean. The estimated N deposition to the whole ECS was 390 Gg N yr-1; this is comparable to the discharge from the Yangtze River to the ECS, indicating the significant contribution of atmospheric deposition. Based on the high-resolution modeling over the ECS, a tendency of high deposition in the western ECS and low deposition in the eastern ECS was found, and a variety of deposition processes were estimated. The dry deposition of coarse-mode NO3- and wet deposition of fine-mode NH4+ were the main factors, and the wet deposition of fine-mode NO3- over the northeastern ECS and wet deposition of coarse-mode NO3- over the southeastern ECS were also found to be significant processes determining N deposition over the ECS.
  Anecdotal and research evidence suggests that individuals with dissociative symptoms exhibit hyperassociativity, which might explain several key features of their condition. The aim of our study was to investigate the link between dissociative tendencies and hyperassociativity among college students.
 
  The study (n=118) entailed various measures of hyperassociativity, measures of dissociative tendencies, depressive experiences, unusual sleep experiences, cognitive failures, and alexithymia.
 
  We found a positive association between dissociative experiences (i.e., depersonalization) and hyperassociativity specific for associative fluency and associative flexibility tasks (including neutral and valenced material), but not for a remote association task. We also found tentative evidence for cognitive failures and alexithymia explaining the link between hyperassociativity and daytime dissociation and nighttime unusual sleep experiences.
 
  Limitations include the use of hyperassociation tasks limited to verbal associations vs.