https://www.selleckchem.com/products/alw-ii-41-27.html The aim of this work was to explore the ability of cutinase in the decolorization of molasses wastewater. Thermophilic cutinase from Thermobifida alba eliminated 76.1-78.2% of colorants and exhibited the highest decolorization efficiency amongst all of the cutinases tested. Cutinase from Thermobifida alba was immobilized on an affordable and efficient modified chitosan carrier and achieved a decolorization yield of 79.3-81.2%. This cutinase removed 66.3-71.1% of pigments and lasted continuously for 5 days. Importantly, it was also shown to continuously and effectively remove COD and BOD5. Compared to other enzymes, the immobilized cutinase from Thermobifida alba had the advantage of being low-cost and had a high expression level and activity. The results confirmed the decolorization occurred by destroying the conjugated system of melanoidins via an addition reaction by cutinase from Thermobifida alba. Thus, this study contributes a more practical and efficient approach to enzymatic decolorization of molasses wastewater.Production of polyhydroxyalkanoates is an important field in the biorefinery as bio-alternative to conventional plastics. However, its commercialization is still limited by high production cost. In this study, a process with the potential to reduce the production cost of polyhydroxyalkanoates was proposed. Mixed cultures accumulated polyhydroxyalkanoates using volatile fatty acid-rich effluents from waste streams, without pH and temperature control. In addition, the impact of two types of carbon sources was investigated by analyzing the microbial community as well as the polyhydroxyalkanoate accumulation capacity. Mixed cultures successfully adapted to different substrates, consuming the volatile fatty acids in their totality. The phyla Proteobacteria, Bacteroidetes and Firmicutes dominated the bacterial community. The highest polyhydroxyalkanoate content was 43.5% w/w, which is comparable to cont