Robotic guidance might be an alternative to classic stereotaxy for biopsies of intracranial lesions. Both methods were compared regarding time efficacy, histopathological results and complications.
 
  A retrospective analysis enrolling all patients undergoing robotic- or stereotactic biopsies between 01/2015 and 12/2018 was conducted. Trajectory planning was performed on magnetic resonance imaging (MRI). With the Robotic Surgery Assistant (ROSA), patient registration was accomplished using a facial laser scan in the operating room (OR), immediately followed by biopsy. In stereotaxy, patients were transported to the CT for Leksell Frame registration, followed by biopsy in the OR.
 
  The average overall procedure time amounted in robotics to 169min and in stereotaxy to 179min (p=0.005). The difference was greatest for temporal targets, amounting in robotics to 161min and in stereotaxy to 188min (p=0,0007). However, the average time spent purely in the OR amounted in robotics to 140min and in stereotaxy to 113min (p<0.001). In 150 robotic biopsies, diagnostic yield amounted to 98%, in 266 stereotactic biopsies to 91%. Symptomatic postoperative hemorrhages were observed in 3 patients (2%) in robotic biopsy and 7 patients (2,7%) in stereotactic biopsy.
 
  Robotics showed a shorter overall procedure time as there is no need for a transport to the CT whereas the pure OR time was shorter in stereotaxy due to skipping the laser registration process. Diagnostic yield was higher in robotics, most likely due to case selection, complication rates were equal.
 Robotics showed a shorter overall procedure time as there is no need for a transport to the CT whereas the pure OR time was shorter in stereotaxy due to skipping the laser registration process. Diagnostic yield was higher in robotics, most likely due to case selection, complication rates were equal.The genomic profiles of osteosarcoma (OS) patients have been extensively investigated; however, the genetic prognostic biomarkers still remain unclear. In the present study, we analyzed the mutational profiles of pre-treatment primary tumor samples from 33 OS patients using whole exome sequencing (WES). These 33 OS patients were divided into two groups according to clinical outcomes a good prognosis group involving 21 patients with tumor free survival, and a poor prognosis group involving the remaining12 patients who had lung metastases at initial diagnosis. Overall we found that the MAPK signaling pathway may play an important role in determining a good prognosis, while the PI3K-Akt signaling pathway may be an important factor leading to a poor prognosis. Significant differences were observed in the number of somatic copy number alterations, including del (3p), amp (4q), del (7p) and amp (8q), between the two groups. Moreover, significant differences were observed in mutation sites and frequencies between these two groups. The good prognosis group exhibited a significantly higher mutation frequency in somatic JAK-STAT and germline base excision repair pathways than the poor prognosis group. Furthermore, significant difference was also observed in the frequency of potentially actionable alterations between the two groups, suggesting that patients with a poor prognosis potentially have access to a larger number of treatment options. These findings highlight the importance of evaluating genomic disparities in OS, and provide a novel insight into the potential prognostic biomarkers.Alternatives to protect crops against diseases are desperately needed to secure world food production and make agriculture more sustainable. Genetic resistance to pathogens utilized so far is mostly based on single dominant resistance genes that mediate specific recognition of invaders and that is often rapidly broken by pathogen variants. Perturbation of plant susceptibility (S) genes offers an alternative providing plants with recessive resistance that is proposed to be more durable. S genes enable the establishment of plant disease, and their inactivation provides opportunities for resistance breeding of crops.  https://www.selleckchem.com/products/Rapamycin.html  However, loss of S gene function can have pleiotropic effects. Developments in genome editing technology promise to provide powerful methods to precisely interfere with crop S gene functions and reduce tradeoffs.This study aimed to map the spatial patterns of Zn in urban topsoil by using multisource geospatial data and machine learning method. Geological map, digital elevation models, and Landsat images were used to extract data related to geology, relief, and land use types and a vegetation index. Urban functional types were derived from the fusion of Systeme Probatoire d'Observation de la Terre 5 images, points of interest, and real-time Tencent user data. A geodetector was adopted to select key environmental covariates. Random forest (RF) and geographically weighted regression (GWR) were employed to model and map Zn concentrations in urban topsoil. The results showed that urban functional type, geology, NDVI, elevation, slope, and aspect were key environmental covariates. Compared with land use types, urban functional types could better reflect the spatial variation in Zn. The RF and GWR models were established using the key environmental covariates, with leave-one-out cross-validated R values of 0.68 and 0.58 and root mean square errors of 0.51 and 0.57, respectively. The results indicated that digital mapping of Zn in urban topsoil by using multisource geospatial data and RF was feasible. RF might be more suitable to fit the stochastic characteristics of Zn in urban topsoils than GWR, which considers deterministic trends in modeling.The influences of polyether sulfone (PES) microplastics and different structures aromatic carboxylic acids such as benzoic acid (BA), phthalic acid (PA), hemimellitic acid (HA), and 1-naphthoic acid (1-NA) on the performances and characteristics of anaerobic granular sludge as well as the microbial community were investigated. The chemical oxygen demand (COD) removal efficiency was the highest in the experimental group with 40 mg/L BA, reaching 90.1%. The inhibitory effect of aromatic carboxylic acids addition on the 2,3,5-triphenyltetrazolium chloride (TTC) activity was more obvious than that on 2-para (iodo-phenyl)-3(nitrophenyl)-5(phenyl) tetrazolium chloride (INT) activity. Compared with the control group (only 0.5 g/L PES microplastics, 60.6 mg TF·g TSS·h-1), the inhibition effect of TTC activity was 32.5 mg TF·g TSS·h-1 and 44.3 mg TF·g TSS·h-1 in the 40 mg/L HA and 40 mg/L 1-NA experimental groups, respectively. When aromatic carboxylic acids were added, the activities of acetate kinase and coenzyme F420 in the anaerobic granular sludge decreased.