05 and 25 ppm. Besides, the SeNPs showed a low cytotoxic effect when cultured with human dermal fibroblasts cells at a range of concentrations up to 1 ppm while showing an anticancer effect toward human melanoma and glioblastoma cells at the same concentration range. This article therefore introduces the possibility of using totally naked SeNPs synthesized by a new PLAL protocol as a novel and efficient nanoparticle fabrication process for biomedical applications. Copyright © 2020 American Chemical Society.G-protein-coupled receptors (GPCRs) are seven transmembrane spanning receptors that regulate a wide array of intracellular signaling cascades in response to various stimuli. To do so, they couple to different heterotrimeric G proteins and adaptor proteins, including arrestins. Importantly, arrestins were shown to regulate GPCR signaling through G proteins, as well as promote G protein-independent signaling events. Several research groups have reported successful isolation of exclusively G protein-dependent and arrestin-dependent signaling downstream of GPCR activation using biased agonists or receptor mutants incapable of coupling to either arrestins or G proteins. In the latter category, the DRY mutant of the angiotensin II type 1 receptor was extensively used to characterize the functional selectivity downstream of AT1AR. In an attempt to understand histamine 1 receptor signaling, we characterized the signaling capacity of the H1R DRY mutant in a panel of dynamic, live cell biosensor assays, including arrestin recruitment, heterotrimeric G protein activation, Ca2+ signaling, protein kinase C activity, GTP binding of RhoA, and activation of ERK1/2. Here, we show that both H1R DRY mutant and the AT1AR DRY mutant are capable of efficient activation of G protein-mediated signaling. Therefore, contrary to the common belief, they do not constitute suitable tools for the dissection of the arrestin-mediated, G protein-independent signaling downstream of these receptors. Copyright © 2020 American Chemical Society.An inorganic sol-gel polymerization process was used as a cross-linking reaction during three-dimensional (3D) bioprinting of cell-containing hydrogel scaffolds. Hybrid hydroxypropyl methyl cellulose (HPMC), with a controlled ratio of silylation, was prepared and isolated as a 3D-network precursor. When dissolved in a biological buffer containing human mesenchymal stem cells, it yields a bioink that can be printed during polymerization by extrusion.  https://www.selleckchem.com/products/rg-7112.html  It is worth noting that the sol-gel process proceeded at pH 7.4 using biocompatible mode of catalysis (NaF and glycine). The printing window was determined by rheology and viscosity measurements. The physicochemical properties of hydrogels were studied. Covalent functionalization of the network can be easily performed by adding a triethoxysilyl-containing molecule; a fluorescent hybrid molecule was used as a proof of concept. Copyright © 2020 American Chemical Society.In this study, we investigated the gauge factor and compressive modulus of hybrid nanocomposites of exfoliated graphite nanoplatelets (xGnP) and multiwalled carbon nanotubes (MWCNTs) in a polydimethylsiloxane matrix under compressive strain. Mechanical and electrical tests were conducted to investigate the effects of nanofiller wt %, the xGnP size, and xGnPMWCNT ratio on the compressive modulus and sensitivity of the sensors. It was found that nanofiller wt %, the xGnP size, and xGnPMWCNT ratio significantly affect the electromechanical properties of the sensor. The compressive modulus increased with an increase in the nanofiller wt % and a decrease in the xGnP size and xGnPMWCNT ratio. However, the gauge factor decreases with a decrease in the nanofiller wt % and xGnP size and an increase in the xGnPMWCNT ratio. Therefore, by investigating the piezoresistive effects of various factors for sensing performance, such as wt %, xGnP size, and xGnPMWCNT ratio, the concept of one- and two-dimensional hybrid fillers provides an effective way to tune both mechanical properties and sensitivity of nanocomposites by tailoring the network structure of fillers. Copyright © 2020 American Chemical Society.The corrosion inhibition performance of propanediyl-1,3-bis(N,N-dimethyl-N-dodecylammonium bromide) and propanediyl-1,3-bis(N,N-dihydroxyethyl-N-dodecylammonium bromide), abbreviated as PDDB and PDHDB, respectively, for carbon steel in 1.0 mol·L-1 hydrochloric acid solution was investigated using the gravimetric method and various electrochemical techniques, together with scanning electron microscopy and energy-dispersive spectrometry. Results show that PDHDB always has a better inhibition performance relative to PDDB, which can be attributed to the introduction of hydroxyl groups at the hydrophilic headgroups, thereby causing an extra interaction between inhibitors and the metal surface and favoring its adsorption. These findings highlight that the modification to the headgroups of Gemini-type inhibitors may be another effective approach to improving their inhibition performance. Copyright © 2020 American Chemical Society.The first stage of any phase transition is a dynamic coupling of transport processes and thermodynamic changes. The free energy change of the phase transition must be negative and large enough to also overcome the penalty work needed for giving space to the new phase. The transition from an unstable situation over to a stable growth is called nucleation. Hydrate formation nucleation can occur along a variety of different routes. Heterogeneous formation on the interface between gas (or liquid) and water is the most commonly studied. A hydrate can also form homogeneously from dissolved hydrate formers in water, and the hydrate can nucleate toward mineral surfaces in natural sediments or a pipeline (rust). A hydrate particle's critical size is the particle size needed to enter a region of stable growth. These critical sizes and the associated nucleation times are nanoscale processes. The dynamics of the subsequent stable growth can be very slow due to transport limitations of hydrate-forming molecules and water across hydrate films.