https://www.selleckchem.com/products/pp1.html 5%. The 162 RC restorations showed a survival of 100% and a total failure rate of 25.3%. RC restorations exhibited more material fractures (p=0.020) and higher discoloration rates (p < 0.001). Pressed LS single-tooth restorations showed lower long-term failure rates than restorations made of RC. Despite the limitations of the clinical observations, single-tooth restorations of both materials can be recommended for permanent use in patients with severe tooth wear. Despite the limitations of the clinical observations, single-tooth restorations of both materials can be recommended for permanent use in patients with severe tooth wear.Acinetobacter johnsonii is one of the major food-spoilage bacteria and can survive under cold stress. In this study, the membrane composition, membrane permeability, and energy transduction of A. johnsonii XY27 cultured at 4 and 30 °C were examined comparatively by flow cytometry combined with liquid chromatography-mass spectrometry and gas chromatography-mass spectrometry. The Na+/K+ATPase activity, alkaline phosphatase and ATPase activity, fluorescence intensity, and cell viability in A. johnsonii XY27 increased with the decrease in cultivation temperature. The polyunsaturated fatty acid and monounsaturated fatty acids have a higher content in A. johnsonii XY27 cultured at 4 °C compared to that cultured at 30 °C, in which the contents of methyl palmitoleate, methyl myristoleate, and methyl oleate increased dramatically with decreasing temperature. Comparative proteomics analysis revealed that 31 proteins were downregulated and 4 proteins were upregulated, in which catalase-peroxidase 1 and cold shock proteins as biomarker proteins could effectively control A. johnsonii during cold adaptation. There are notable disparities by race/ethnicity in the sexual health of US adolescents and young adults. Our objective was to examine change over time in racial-ethnic disparities in sexual behaviours amo