After treatment completion, families living in low socioeconomic areas commonly reported ongoing financial difficulties. Mothers, particularly those who were on maternity leave when their child was diagnosed with cancer, reported ongoing employment impacts including unemployment. CONCLUSIONS Clinical staff including social workers could more consistently assess families' financial distress and refer to professional services who can offer guidance for financial decision-making as standard care. Flexible workplace agreements appear important for parents of children with cancer. Our findings can assist organizations to understand that cancer-related disruptions are likely to continue after treatment completion, and therefore should offer benefits to parents where possible. © 2020 Wiley Periodicals, Inc.Six new eudesmane-type sesquiterpene derivatives, artemargyinins A-F ( 1 - 6 ) were isolated from the leaves of Artemisia argyi . Their structures were elucidated based on the extensive analysis of spectroscopic data. Compounds 1 - 6 feature a lactone ring-opening eudesmane-type sesquiterpene with an isoprenoid group at C(8). All compounds were tested for their inhibitory effects on lipopolysaccharide-induced nitric oxide (NO) production in RAW264.7 macrophages.Compounds 1 - 6 showed more potent NO production inhibitory activity with IC 50 values ranging from 7.66 ± 0.53 to 61.19 ± 2.54 μ M than the positive control quercetin (IC 50 = 74.34 ± 1.39 μ M). Among them, 3 and 4 exhibited strong inhibitory activity with IC 50 values of 8.08 ± 0.21 and 7.66 ± 0.53 μ M, respectively. © 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.While packed bed chromatography, known as conventional chromatography, has been serving the biopharmaceutical industry for decades as the bioseparation method of choice, alternative approaches are likely to take an increasing leading role in the next few years. The high number of new biological drugs under development, and the need to make biopharmaceuticals widely accessible, has been driving the academia and industry in the quest of anything but conventional chromatography approaches. In this perspective paper, these alternative approaches are discussed in view of current and future challenges in the downstream processing field. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.BACKGROUND VTP-50469 is a potent inhibitor of the menin-MLL1 interaction and is implicated in signaling downstream of EWSR1-FLI1. PROCEDURE VTP-50469 was evaluated against seven Ewing sarcoma (EwS) xenograft models and in vitro against EwS cell lines. RESULTS VTP-50469 showed limited antitumor activity, statistically significantly slowing tumor progression in four tumor models but with no evidence of tumor regression. In vitro, the IC50 concentration was 10 nM for the mixed lineage leukemia (MLL)-rearranged leukemia cell line MV4;11, but > 3 μM for EwS cell lines. CONCLUSIONS In contrast to its high level of activity against MLL1-rearranged leukemia xenografts, VTP-50469 shows little activity against EwS models. © 2020 Wiley Periodicals, Inc.OBJECTIVES To examine the association of lipoprotein lipase (LPL) polymorphisms with cardiovascular disease (CVD) risk factors in European adolescents, along with the influence of physical activity on these associations.  https://www.selleckchem.com/pharmacological_epigenetics.html  METHODS A total of 13 LPL polymorphisms were genotyped in 1.057 European adolescents (12-18 years old) from the Healthy Lifestyle in Europe by Nutrition in Adolescence Cross-Sectional Study. Serum lipids, glucose, insulin and leptin levels were measured and a CVD risk score was computed. We also measured body weight and height, waist and hip circumferences, and triceps and subscapular skinfold thickness. Physical activity was objectively measured by accelerometry for 7 days. RESULTS The rs1534649, rs258, rs320 and rs328 polymorphisms were associated with several CVD risk factors (i.e., body mass index [BMI], Triglycerides [TG], Leptin, and cholesterol/High density lipoprotein [HDL], Low density lipoprotein [LDL]/HDL, TG/HDL ratios). TG and TG/HDL were associated with haplotype blocks 3 (rs282, rs285 polymorphisms) and 4 (rs3126, rs320, rs328, rs10099160 polymorphisms), being the latter also associated with the CVD risk score. Physical activity modulated the association of adiposity with rs1534649 and rs258 polymorphisms. CONCLUSIONS Polymorphisms rs1534649, rs258, rs320 and rs328, and two haplotypes of LPL were significantly associated with CVD risk factors in European adolescents. Higher levels of moderate to vigorous physical activity may attenuate the effects of rs1534649 and rs258 polymorphisms on adiposity. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.OBJECTIVES Government guidance promote benchmarking comparing quality of care including both clinical values and patient reported outcome measures in young persons with Type 1 diabetes (T1D). The aim was to test if the Nordic DISABKIDS® Health-related Quality-of-Life (HrQoL) modules were construct valid and measurement comparable within the three Nordic countries. METHODS Data from three DISABKIDS® validation studies in Sweden, Denmark and Norway were compared using Rasch and the graphical log linear Rasch modeling. Monte Carlo methods were used to estimate reliability coefficient and target was defined as the point with the lowest standard error of the mean. Self-report data were available from 99 Danish (8-18 years), 103 Norwegian (7-19 years) and 131 Swedish (8-18 years) young people. RESULTS For the DISABKIDS® higher scores on most subscales were noted in the Norwegian population. The Swedish sample had a significantly higher score on the "Diabetes treatment" subscale and scores closer to optimal target than the other countries. For each country construct validity and sensitivity were acceptable when accounting for differential item function (DIF) and local dependency (LD). Less LD and DIF were found if only Denmark and Norway were included. The combined model was reliable however, some differences were noted in the scale translations relating to the stem and response alternatives, which could explain the discrepancies. CONCLUSION The Nordic versions of the DISABKIDS® questionnaires measures valid and reliable HrQoL both within and between countries when adjusted for DIF and LD. Adjusting the Likert scales to the same respond categories may improve comparability. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.