Thiolated-alloy nanocluster Au15.37Cu16.63(S-Adm)20 ((AuCu)32 for short) has been synthesized. Single crystal X-ray crystallography (SC-XRD) proved that this cluster contains a Au14Cu6 core, which is composed of double superatom chains, two M4(SR)5 (M = Au/Cu) motifs, four Cu(SR)2 monomer staples and two SR molecules at the waist. The composition of this nanocluster is confirmed by SC-XRD and further verified by XPS, EDX and 2H-NMR. Surprisingly, double superatom chains connect to each other only via two Au-Au bonds, which makes the kernel resemble a tunnel. Combined with DFT calculation and electronic structure analysis, it is further proved that the (AuCu)32 nanocluster contains six 2e alloy superatomic Au3Cu2+ units. This work is the first report showing that superatom units (CuAu32+) self-assembling to a hollow kernel maintain the superatom characteristic of metal nanoclusters, which enriches the fundamental knowledge of metal superatom clusters. The stable electronic structure of the nanocluster provides an experimental basis for theoretical analysis in the future. In addition, the hollow structure may be promising in catalytic applications.Ultra low PtPd alloy deposited on Ni12P5 nanostructures (PtPd/Ni12P5) exhibited enhanced ORR activity (onset 1.003 V and E1/20.95 V) on par with commercial Pt/C and superior OER activity with 81% reduction of the precious metal compared to the commercial catalyst.We find that luminol can react with artemisinin (ART) to produce chemiluminescence (CL) in the absence of a catalyst and ascorbic acid (AA) can quench luminol-ART CL. Based on its efficient inhibition effect on luminol-ART CL, a new AA detection method is established. The calibration curve for the determination of AA is in the linear range of 5 × 10-7 M to 1 × 10-4 M with a detection limit of 50 nM, which is more sensitive than many other reported methods. This CL approach was utilized to detect AA in vitamin C tablets by applying the standard addition method, and the recoveries of 104.0%, 96.8% and 103.4% were obtained, respectively, at concentrations of 1 μM, 5 μM and 10 μM with a RSD value of less than 3.6%. This developed method for AA assay is distinguished by its fastness, reproducibility, easy operation and good selectivity.In this study, a gold labelled immunochromatographic assay was developed to detect tigecycline (TGC) in human serum. For this purpose, an anti-TGC monoclonal antibody, 2G7, was produced and characterized, and was found to have a 50%-inhibitory concentration (IC50) of 2.303 ng mL-1 and a limit of detection (LOD) of 0.215 ng mL-1 for TGC. This strip assay had a visual limit of detection (vLOD) of 50 ng mL-1 and a cut-off value of 1000 ng mL-1 for TGC in human serum, when assessed by eye. With the aid of a strip scan reader, a quantitative determination of TGC was obtained with a calculated limit of detection (cLOD) of 15.03 ng mL-1. Analysis of TGC in human serum indicated that the results of our strip assay compared well with results obtained using ic-ELISA and LC-MS/MS. Therefore, this strip assay represents a sensitive and reliable method for the on-site detection of TGC in serum samples.This work describes a new method for the analysis of handwritten documents through a system composed of a pre-selector optical analyser equipped with light sources of different wavelengths coupled with bandpass filters combined with an optical coherence tomography (OCT) instrument. The optical analyser identifies regions with different pen pressures on the paper using specific wavelengths from ultraviolet (UV) to infrared (IR) and bandpass filters. Then the selected regions are analysed with a coherence tomography analyser to measure the depth of grooves and capture three-dimensional images. With this methodology, it is possible to identify similarities, or differences, between the pieces of evidence under investigation, increasing the possibility of correct attribution concerning the authorship of the signature and we also showed that this feature is independent of the paper substrate. In this work, a new strategy will be presented to categorize and quantify pen pressure in order to aid a better response for a forensic examiner. Thereby, from the observed areas that display higher pressures (more significant grooves), it is possible to determine the authorship of the signature.A chemical-chemical redox cycling amplification strategy was introduced into a photocathodic immunosensing system. To prove the applicability of the method, a novel self-powered photochemical system by integrating the photoanode and photocathode was designed for protein analysis.Aptamers, which are called chemical antibodies for their high affinity and specificity to targets, have great potential as analytical tools to detect pesticides. In this work, a DNA aptamer for thiamethoxam was isolated by an improved SELEX (systematic evolution of ligands by exponential enrichment) strategy, in which the ssDNA library was fixed on streptavidin-agarose beads through a short biotin labeled complementary strand. After 13 rounds of selection, the random ssDNA pool was successfully enriched.  https://www.selleckchem.com/products/leupeptin-hemisulfate.html  Three sequences were chosen as aptamer candidates through sequencing and analysis and were transformed into fluorescent probes to evaluate their interactions with thiamethoxam. A fluorescent turn-on aptasensor for thiamethoxam based on the best aptamer (FAM-Thi13) and a short quenching strand were further designed and showed a quantitative linear range from 10 to 1000 nM with a detection limit of 1.23 nM for thiamethoxam. Molecular docking and molecular dynamics were used to investigate the binding site of the main probe of the aptasensor (FAM-Thi13) and thiamethoxam. Satisfactory results were also obtained in quantifying thiamethoxam in environmental water samples by the developed fluorescent aptasensor.We generated stable amphiphilic copolymer-based polymeric micelles (PMs) with temperature-responsive properties utilizing Pluronic® L35 and a variety of ionic liquids (ILs) to generate different aqueous two-phase micellar systems (ATPMSs). The partitioning of the hydrophobic model compound curcumin (CCM) into the PM-rich phase and the drug delivery capabilities of the PMs were investigated. ATPMSs formed using more hydrophobic ILs (i.e., [Ch][Hex] ≈ [Ch][But] > [Ch][Pro] > [Ch][Ac] ≈ [Ch]Cl) were the most effective in partitioning (KCCM) and recovering (RECRich) CCM into the PM-rich phase (15.2 less then KCCM less then 22.0 and 90% less then RECRich less then 95%, respectively). Moreover, using 1.2 M [Ch][But] and 0.2 M [Ch][Hex] ILs yielded higher encapsulation efficiency (EE) (94.1 and 96.0%, respectively) and drug loading (DL) capacity (14.8 and 16.2%, respectively), together with an increase in the average hydrodynamic diameter of the PMs (DH) (42.5 and 45.6 nm, respectively). The CCM-PM formulations were stable at 4.