https://www.selleckchem.com/products/h-1152-dihydrochloride.html B. brevis GZDF3, a gram-positive bacterium, was a plant growth-promoting rhizosphere bacterium (PGPR) isolated from the rhizosphere soil of Pinellia ternate (an important Chinese Traditional Medicinal herb). The GZDF3 strain produced certain active compounds, such as siderophore, which were the final metabolite products of non-ribosomal peptide synthetase (NRPS) and independent non-ribosomal peptide synthetase (NIS) activity. The present study attempted to investigate the siderophore production characteristics and conditions by Bacillus sp. GZDF3. And the antibacterial activity of siderophore on pathogenic fungi was also investigated. The optimal conditions for the synthesis of siderophore were determined by single factor method, sucrose 15 g/L, asparagine 2 g/L, 32 °C and, 48 h. The optimized SA（Sucrose-Asparagine Medium）medium significantly increased the production of siderophore, from 27.09% to 54.99%. Effects of different kinds of metal ions on siderophore production were explored here. It showed that Fe3+ and Cu2+ significantly inhibited the synthesis of siderophore. The preliminary separation and purification of siderophore by immobilized metal affinity chromatography (IMAC) has strong antibacterial activity against Candida albicans. The synergistic effect of siderophore and Amphotericin B was demonstrated. Our results have shown that the GZDF3 strain could produce a large amount of siderophore and has strong antagonistic activity, which is helpful in the development of new biological control agents.The purpose of this study was to identify strawberry wilt pathogen and evaluate the efficacy of Chlorella fusca CHK0059 for improving plant growth and suppressing Fusarium wilt. We identified 10 isolates of wilt pathogens of non-pesticide Seolhyang strawberry plant, including Fusarium oxysporum f. sp. fragariae using morphological and molecular analysis. On the 15th day after 0.4% CHK0059 treatment, t