Cardiac fibrosis is associated with fine particulate matter (PM2.5) exposure. In addition, whether high-fat diet (HFD) could exacerbate the PM2.5-induced cardiac injury was unevaluated. Thus, this study was aimed to investigate the combined effects of PM2.5 and HFD on cardiac fibrosis. The echocardiography and histopathological analysis showed that co-exposure of PM2.5 and HFD had a significant deleterious effect on both cardiac systolic and diastolic function accompanied the myofibril disorder and myocardial fibrosis in C57BL/6 J mice than exposed to PM2.5 or HFD alone.  https://www.selleckchem.com/products/ga-017.html  The augmented oxidative damage and increased α-SMA area percentage were detected in heart tissue of mice exposed to PM2.5 and HFD together. PM2.5 upregulated the expressions of cardiac fibrosis-related special markers, including collagen-I, collagen-III, TGF-β1, p-Smad3 and total Smad3, which had more pronounced activations in co-exposure group. Meanwhile, the factorial analysis exhibited the synergistic interaction regarded to the combined exposure of PM2.5 and HFD. Simultaneously, PM2.5 and palmitic acid increased intracellular ROS generation and activated the TGF-β1/Smad3 signaling pathway in cardiomyocytes. While the ROS scavenger NAC had effectively attenuated the ROS level and suppressed the TGF-β1/Smad3 signaling pathway. Taken together, our results demonstrated combined exposure to PM2.5 and HFD could aggravate cardiac fibrosis via activating the ROS/TGF-β1/Smad3 signaling pathway. BACKGROUND Banning smoking from public places may prevent adolescent smoking, but there is little evidence on impact of smoke-free policies (SFPs) from low and middle-income countries. This study assessed to what extent the adoption of local SPFs in Indonesia between 2007 and 2013 associated with adolescent smoking. METHODS Data on 239,170 adolescents (12-17 years old) were derived from the 2007 and 2013 national health surveys in 497 districts and 33 provinces. This study compared 2013 survey respondents living in districts/provinces that adopted SFPs between 2007 and 2013, with 2007 respondents and 2013 respondents in districts/provinces that did not adopt policies. Multilevel logistic regression analysis assessed whether SFP was associated with daily and non-daily smoking. We controlled for survey year, SFP in 2007, socio-demographics, and district characteristics. RESULTS Strong district SFPs was significantly associated with lower odds of daily smoking (OR0.81, 95 %CI0.69-0.97), but non-significantly with non-daily smoking (OR0.89, 95 %CI0.76-1.05). Strong provincial SFPs was not associated with daily smoking (OR1.02, 95 %CI0.84-1.25), but was associated with higher odds of non-daily smoking (OR1.22, 95 %CI0.99-1.51). Moderately strong SFPs did not consistently show associations in the same direction. For example, moderately strong provincial SFP was associated with higher odds of daily smoking (OR1.27, 95 %CI1.11-1.46) and lower odds of non-daily smoking (OR0.82, 95 %CI0.72-0.93). CONCLUSION We did not detect a consistent short-term effect of district and province-level smoke-free policies on adolescent smoking in Indonesia. Weak implementation and poor compliance may compromise effectiveness, which would call for improvement of SFP implementation in Indonesia. With the proposed Circular Economy Package, the European Union is striving to play a leading role in the implementation of recycling goals. The significantly increased recycling targets are just some of the defined objectives. However, to assess the Member States' attainment of the new recycling targets, the European Union still builds on a purely quantitative recycling rate assessment procedure that neglects to include qualitative recycling aspects. This circumstance could lead to additional quality losses in recycling processes because recyclers might tend to focus exclusively on higher quantities to achieve the stricter recycling targets on time. To prevent such a development, the aim of this study is to establish a complementary recycling indicator that combines quantitative and qualitative recycling aspects in one single metric. The basis of this assessment method is the statistical entropy approach, which enables the concentrating or diluting effect of a recycling process brought about through the separation or mixing of materials to be measured. The results of the statistical entropy metric will provide greater insight into recycling processes (or systems) and thereby yield enhanced information on the quantity and purity of recycling outputs. The simple structure of the new approach will allow enhanced comparisons between technologies as well as national recycling performance. A case study on plastic packaging recycling demonstrates that the new recycling indicator provides multifaceted findings relative to the hitherto purely quantitative recycling assessment data, hence enriching conclusions on the recycling performance. We investigated the distribution of 18 elements including non-volatiles (Al, P, Ca, Fe, Mg, K, Mn, Cu, Na, Cr, and Ni), semi-volatiles (Zn, Pb, Ag, As, and Cd), and volatiles (Hg and S) and compared their behaviors in two types of full-scale sewage sludge mono-incinerators, namely, a step-grate stoker (GS) and two fluidized bed incinerators (F-types), with the same feed sludge. Most of the non-volatile elements were enriched five-fold in all incinerated sewage sludge ash (ISSA), while the volatile S and Hg were barely enriched in ash due to the combustion components generated in the gas phase. While the semi-volatile elements were also enriched five-fold in the F-types, a different enrichment behavior was observed in the GS. Boiler and multi-cyclone dust in the GS showed higher enrichments of Pb and Cd compared to ash due to the combined effects of lower temperature and smaller particle size. Compared to the F-types, the GS generated ashes with lower toxicity as the major component (99.7%) and hazardous dust as the minor component. In the future, more attention should be paid to grate stokers in terms of recycling ISSA. Bayleyite is a highly hydrated uranyl tricarbonate mineral containing eighteen water molecules per formula unit. Due to this large water content, the correct description of its crystal structure is a great challenge for the first principles solid state methodology. In this work, the crystal structure, hydrogen bonding, mechanical properties and infrared spectrum of bayleyite, Mg2[UO2(CO3)3] · 18 H2O, have been investigated by means of Periodic Density Functional Theory methods using plane wave basis sets and pseudopotentials. The computed unit-cell parameters, interatomic distances, hydrogen bonding network geometry and the X-ray powder diffraction pattern of bayleyite reproduce successfully the experimental data, thus confirming the crystal structure determined from X-ray diffraction data. From the energy-optimized structure, the elastic properties and infrared spectrum have been determined using theoretical methods. The calculated elastic properties include the bulk modulus and its pressure derivatives, the Young and shear moduli, the Poisson ratio and the ductility, hardness and anisotropy indices.