https://www.selleckchem.com/products/n-acetyl-dl-methionine.html Altogether, we achieved up to 92% grafting success in A. thaliana. Reconnection of vasculature was demonstrated by transport of a vasculature-specific dye across the grafting site. Phloem and xylem reconnection were completed 3-4 and 4-6 days after grafting, respectively, in a temperature-dependent manner. We observed that plants with grafted cotyledons match plants with intact cotyledons in biomass production and rosette development. Conclusions This cut and paste cotyledon-to-petiole micrografting protocol simplifies the handling of plant seedlings in surgery, increases the number of grafted plants per hour and greatly improves success rates for A. thaliana seedlings. The developed cotyledon micrografting method is also suitable for other plant species of comparable size. © The Author(s) 2020.Background Variable-rate fertilization is crucial in the implementation of precision agriculture and for ensuring reasonable and efficient fertilizer application and nutrient management that is tailored to local conditions. The overall goal of these technologies is to maximize grain output and minimize fertilizer input and, thus, achieve the optimal input-output production ratio. As the main form of variable-rate fertilization, real-time variable-rate control technology adjusts fertilizer application according to the growth status and nutrient information of crops and, as such, its effective application relies on the stable and accurate acquisition of crop phenotypic information. Results Due to the relationship between crop phenotype and real-time fertilizer demand, phenotypic information has been increasingly applied in these contexts in recent years. Here, the establishment and characteristics of inversion models between crop phenotypic information and nutritional status are reviewed. The principles of real-time monitoring applications, the key technologies relating to crop phenotypic biological parameters, an