https://www.selleckchem.com/ reinforced indicator-based sustainability framework will make a core, not only to assess existing plants with various characteristics but also for new refineries that are going to be developed based on sustainability principles.Although the macroinvertebrates have been widely used as bio-indicator for river water quality assessment in developed countries, its application is new in Iran and data on the health status of the most ecologically important rivers in Iran is scarce. The present study aimed at monitoring and assessing the ecological quality of Aghlagan river, northwest of Iran, using integrated physicochemical-biological approaches. A total of 14,423 samplings were carried out from the headwater to downstream sites at four stations (S1, 2, 3, 4) by a Surber sampler (30 cm × 30 cm) from June 2018 to April 2019. The results obtained from macroinvertebrate biotic index revealed that the genera of Gammarus (Amphipoda) and Baetis (Ephemeroptera) were the most abundant in all seasons. The PAST software was applied to analyze the diversity indices (Shannon-Weiner diversity, Evenness, and Simpson indices). Based on the cluster analysis, S3 established the least similarity to other stations. The average frequency of each macroinvertebrate species was determined by one-factor analysis of similarities (ANOSIM). In accordance with canonical correspondence analysis (CCA), temperature and phosphate were found as the dominant factors effecting the macroinvertebrate assemblage and distribution. Moreover, the results obtained from the biological indices concluded very good quality of S4 by Helsinhoff and EPT indices and fair quality using BMWP index. The data on the macrobenthos assemblage and dynamics in the Aghlagan river across a hydraulic gradient provided useful information on water management efforts that assist us to find sustainable solutions for the enhanced quality of the river by balancing environmental and human values.In this study,