https://www.selleckchem.com/products/eidd-2801.html At the same time, and were down-regulated in B80. Results of LC-MS also showed that epicatechin was not detected in seeds of B80. We validated the accuracy of our RNA-seq data by RT-qPCR of nine critical genes. Epicatechin was not detected in seeds of B80 by LC-MS/MS. The expression levels of flavonoid biosynthetic pathway genes and the relative content of flavonoid biosynthetic pathway metabolites clearly explained yellow seed color formation in . This study provides a foundation for further research on the molecular mechanism of seed coat color formation. The expression levels of flavonoid biosynthetic pathway genes and the relative content of flavonoid biosynthetic pathway metabolites clearly explained yellow seed color formation in B. rapa. This study provides a foundation for further research on the molecular mechanism of seed coat color formation.Despite considerable tolerance to salt and alkali stress, Leymus chinensis populations on the southwestern Songnen Plain in northern China are threatened by increasing soil salinity and alkalinity. To explore the species' responses to saline-alkali stress, we grew it in substrates with varying concentrations of nitrogen (N) and phosphorus (P) while applying varying levels of saline-alkali stress (increasing in 14-, 17- or 23 -day intervals). We measured the plants' contents of N and P, and the NP ratio, and calculated their homeostasis indices (HN , HP and HNP ) under each nutrient and saline-alkali stress treatment. The N content was found to be more sensitive to saline-alkali stress than the P content. The N and P contents were highest and the NP ratio was stable at pH 8.4. At both pH 8.1 and 8.4, H NP> H N > H P, but the indices and their relations differed at other pH values. Exposure to saline-alkali stress for the 14-day incremental interval had weaker effects on the plants. Rapid changes in salinity-alkalinity weakened both the positive effects of the weakly a