he safety profile of the two treatments; both were associated with lower risks of revision surgery than previously reported. Cite this article Bone Joint J 2021;103-B(2)256-263.Two new sucrose derivatives, named as cannadica A (1) and cannadica B (2), and eight known compounds, 6'-O-acetyl-3-O-(E)-p-coumaroylsucrose (3), heterophylloside C (4), 6'-O-vanilloylarbutin (5), isotachioside (6), 2,6-dimethoxy-p-hydroquinone 1-O-β-D-glucopyranoside (7), 3,5-dimethoxy-p-hydroquinone 1-O-β-D-glucopyranoside (8), benzyl glucoside (9), and 3,4-dihydroxybenzaldehyde (10) were isolated from the roots of Canna indica L. by various chromatographic methods. Their structures were established by extensive spectroscopic analysis (UV, IR, HR-ESI-MS and NMR) and by comparison of the spectral data with those reported in the literature. Compounds 1-10 were evaluated their antioxidant activity by peroxyl radical absorbance capacity assay. Compounds 4 and 5 exhibited the most peroxyl radical absorbance capacity. At concentration of 1 µM, their ORACROO* values were 4.86 ± 0.39 and 3.11 ± 0.26, respectively, fold-up to that of trolox as an internal standard.1. Plant extracts and oils are supplemented in diets for chickens due to their antimicrobial capacities; however, little information exists whether they influence intestinal motility and barrier function.2. The present study aimed to determine the effect of increasing levels of cinnamon bark oil (CBO; 0%, 0.038%, 0.076% and 0.151%) and coconut oil emulsions prepared with soy and sunflower lecithin on the contractile function of enteric wall muscles in the jejunum and ileum and jejunal barrier function in laying hens.3. For testing muscle contraction, mid-jejunal and ileal segments (n = 4 each per hen) from four laying hens were placed in a longitudinal orientation into isolated organ baths filled with Krebs buffer and fastened to force transducers. Muscle segments were induced to contract with acetylcholine and the effects of the oil emulsions on contraction were measured.4. For barrier function, distal jejunal pieces were stripped of serosa before mounting into Ussing chambers and recording changes in short-circuit current (ISC) and transepithelial tissue conductivity (GT) before and after addition of the respective emulsion.5. The CBO decreased the muscle tone, representing a relaxation of on average 36.2% and 42.6% for the jejunum and ileum, respectively, compared to before the addition (P less then 0.001). Moreover, CBO linearly decreased the ISC and GT of the jejunal mucosa, indicating a greater absorption of anions and increased barrier function (P less then 0.001). Only the coconut oil-sunflower lecithin emulsion relaxed the muscles, whereas both coconut oil-lecithin emulsions increased the ISC but reduced the GT of the jejunal mucosa, which suggested an increased cation absorption and decreased paracellular permeability, respectively (P less then 0.05).6. In conclusion, CBO and coconut oil-lecithin emulsions showed the potential to increase jejunal barrier function, whereas CBO may be more efficacious to slow down digesta passage in the small intestine.Two new glycosides, namely panalaside A (1) and panalaside B (2) were isolated from from Panzerina lanata (L.) Soják. The structures of 1 and 2 were elucidated by spectroscopic methods, including UV, IR, HR-ESI-MS and extensive 1 D and 2 D NMR techniques.Purpose To investigate the change and recovery of choroid thickness after short-term application of 1% atropine gel and its influencing factors in 6-7-year-old children.Materials and Methods 71 right eyes of 71 children were enrolled and divided into myopia and control group. 1% atropine gel was administered twice a day for one week and then stopped. Spherical equivalent (SE), accommodative amplitude (AA), keratometry (K), axial length (AL), and choroidal thickness (CT) were obtained at baseline and 1st, 4th, and 8th weeks.  https://www.selleckchem.com/products/Puromycin-2HCl.html  CT was measured at subfovea and 1 mm, 2 mm, and 3 mm temporal, superior, nasal, and inferior from the fovea using spectral-domain optical coherence tomography.Results In both groups, all CTs increased following the change in SE, AA, and AL after administration of 1% atropine for one week. They gradually recovered to baseline levels seven weeks after withdrawal. The change (Δ) in CT at 3 mm superior from the fovea was significantly higher in the myopia group than in the control group. In both groups, ΔCT at subfovea had no significant correlation with SE, AA, and AL, both at baseline and one week. However, ΔCT at subfovea was negatively correlated with ΔAL in the control group.Conclusions One-week application of 1% atropine gel may increase CT in 6-7-year-old Chinese children. Meanwhile, the recovery process after withdrawal lasts seven weeks. During the recovery process, the changes in structural parameters (AL, CT) and functional parameters (AA, SE) in both groups occurred synchronously. The SE, AA, and AL at baseline may not predict the extent of atropine's effect on CT.1. The long noncoding RNA lncGLM is significantly differentially expressed in the livers of peak-laying hens compared with that in the livers of pre-laying hens, but its potential biological role and expression regulation are unclear.2. To explore the potential biological function of lncGLM, single nucleotide polymorphism (SNP) detection and association analysis were carried out in the Gushi×Anka F2 resource population.3. The tissues and spatiotemporal expression characteristics of lncGLM were analysed by real-time quantitative PCR. The effects of 17β-oestradiol on the expression of lncGLM expression were analysed through in vitro and in vivo experiments.4. The results showed that a g.19069338 T > C SNP was present in lncGLM. Association analysis revealed that lncGLM was significantly associated with body slanting length at 12 weeks, body weight at 12 weeks, shank length at four weeks, chest depth at eight weeks, pelvic width at 12 weeks, eviscerated weight, head weight, pancreas weight, pectoralis weight, leg muscle weight, muscular stomach weight rate, pancreas weight rate, carcase weight, aspartate aminotransferase, creatinine and pectoral muscle water loss rate.5. The expression of lncGLM in the liver was higher than that in other sampled tissues. In addition, the expression of lncGLM in the liver was significantly higher in the peak-laying period than at the pre-laying period. Both in vitro and in vivo experiments showed that lncGLM expression was regulated by 17β-oestradiol via oestrogen receptor alpha (ER-α). These results demonstrated that the chicken lncGLM gene is highly expressed in liver tissue and regulated by oestrogen through ER-α.