BACKGROUND Rapid and accurate diagnosis of mucopolysaccharidoses (MPS) is still a challenge due to poor access to screening and diagnostic methods and to their extensive clinical heterogeneity.  https://www.selleckchem.com/products/tpen.html  The aim of this work is to perform laboratory biochemical testing for confirming the diagnosis of mucopolysaccharidosis (MPS) for the first time in Morocco. METHODS Over a period of twelve months, 88 patients suspected of having Mucopolysaccharidosis (MPS) were referred to our laboratory. Quantitative and qualitative urine glycosaminoglycan (GAG) analyses were performed, and enzyme activity was assayed on dried blood spots (DBS) using fluorogenic substrates. Enzyme activity was measured as normal, low, or undetectable. RESULTS Of the 88 patients studied, 26 were confirmed to have MPS; 19 MPS I (Hurler syndrome; OMIM #607014/Hurler-Scheie syndrome; OMIM #607015), 2 MPS II (Hunter syndrome; OMIM #309900), 2 MPS IIIA (Sanfilippo syndrome; OMIM #252900), 1 MPS IIIB (Sanfilippo syndrome; OMIM #252920) and 2 MPS VI (Maroteaux-Lamy syndrome; OMIM #253200). Parental consanguinity was present in 80.76% of cases. Qualitative urinary glycosaminoglycan (uGAGs) assays showed abnormal profiles in 31 cases, and further quantitative urinary GAG evaluation and Thin Layer Chromatography (TLC) provided important additional information about the likely MPS diagnosis. The final diagnosis was confirmed by specific enzyme activity analysis in the DBS samples. CONCLUSIONS The present study shows that the adoption of combined urinary substrate analysis and enzyme assays using dried blood spots can facilitate such diagnosis, offer an important tool for an appropriate supporting care, and a specific therapy, when available.BACKGROUND The current study aims to investigate the expression of miR-587 in platelets of patients with acute coronary syndrome (ACS) and its correlation with the severity of coronary artery disease. METHODS One hundred thirty-nine patients with ACS who underwent coronary angiography (CAG) and hospitalized in the Department of Cardiology of Affiliated Tengzhou Central People's Hospital of Jining Medical University were selected. In addition, 42 subjects with normal CAG results were selected as the control group. The relative expression of platelet miR-587 was analyzed between ACS patients and control subjects. RESULTS The relative expression of platelet miR-587 and Gensini score in acute myocardial infarction (AMI) patients were significantly higher than those in the unstable angina pectoris (UA) and control groups (median (IQR) 1.97 (0.47) vs. 1.49 (0.43) vs. 1.04 (0.35); 65.07 (21.6) vs. 44.58 (28.56) vs. 13.67 (11.8)). Furthermore, the relative level of miR-587 in ACS patients with three vessel lesions was significantly higher than those with double vessel le-sion and single vessel lesion (2.68 (0.64) vs. 1.85 (0.53) vs. 1.22 (0.40)). Additionally, the relative expression of platelet miR-587 in patients with severe coronary artery disease was significantly higher than in those with moderate or mild coronary artery disease (2.44 (0.59) vs. 1.70 (0.49) vs. 0.96 (0.32)). Pearson's correlation analysis showed that the Gensini score and creatine kinase isoenzyme (CK-MB) of ACS patients were positively correlated with the expression of platelet miR-587 (r = 0.785, p = 0.000; r = 0.806, p = 0.000). CONCLUSIONS The expression of platelet miR-587 was closely related to the severity of coronary artery stenosis and could be used as a potential target for diagnosis or prognosis.BACKGROUND Invasive Gram-positive bacilli infections are commonly encountered in immunocompromised patients. In this paper, we report a bacteremia caused by Turicella otitidis in a patient with diffuse large B-cell lymphoma. METHODS Bacteria was identified by VITEK MALDI-TOF MS. Drug sensitivity was analyzed by disk diffusion method. RESULTS MALDI-TOF MS data demonstrated that the infection bacteria was Turicella otitidis. Drug susceptibility data showed that Turicella otitidis was possibly sensitive to vancomycin, polymyxin B, and chloramphenicol. Body temperature of the patient dropped after administration of vancomycin. The data indicated that vancomycin could be used to treat the infections caused by Turicella otitidis. CONCLUSIONS MALDI-TOF MS can be used for the rapid and accurate identification of Turicella otitidis. Vancomycin can be used to treat the infection caused by Turicella otitidis. This study may provide a reference for the diagnosis and treatment of Turicella otitidis.BACKGROUND Do D-dimer levels influence the pregnancy outcomes after treatment with low molecular weight heparin (LMWH) in women of recurrent miscarriage (RM), repeated biochemical pregnancy losses (BPL), and a positive test for antiphospholipid antibodies (aPLs)? METHODS This study was a retrospective chart review of 569 RM patients who were identified as having a history of BPL and a positive aPL. These patients were grouped into three groups according to their treatment plan including those who received low dose aspirin (LDA) alone (group A), LDA plus LMWH after ovulation therapy (group B), and LDA plus LMWH after pregnancy confirmation (group C). We hypothesized that the administration of LMWH after ovulation increased the rates of live birth. D-dimer may predict the pregnancy outcome after treatment. RESULTS The live birth rate of group B and group C is significantly higher than group A (86.96% and 66.80% vs. 52.89%, p less then 0.0001, respectively). The live birth rate in group A, B, and C with elevated D-dimer is 36.92%, 90.52%, and 61.60% respectively. However, there is no significant difference in live birth rate among those who had normal baseline D-dimer. CONCLUSIONS These results suggest that LMWH therapy is more effective in improving the live birth rate when given after ovulation than after pregnancy confirmation. The plasma D-dimer assay can possibly guide LMWH treatment appropriately.BACKGROUND To analyze the difference of the antibiotic resistance of Acinetobacter baumannii (A. baumannii) isolated from our hospital between 2014 and 2017. METHODS We retrospectively evaluated the patients with a confirmed diagnosis of A. baumannii infection at a tertiary general hospital in Guilin during the period between January 2014 and December 2017. The following clinical and demographic data were collected age, gender, specimens, microbiology results, and antibiotic resistance patterns of isolates. Bacterial identification and susceptibility testing were performed using MALDI-TOF MS and VITEK 2 COMPACT systems. The results were analyzed according to the Clinical and Laboratory Standards Institute (CLSI) 2018 definitions. RESULTS From 2014 to 2017, 1,294 strains of A. baumannii were detected, 41.5% of which came from ICU. The sputum separation rate from ICU was significantly higher than those from non-ICU (p less then 0.05). Except for amikacin, levofloxacin, and compound sulfamethoxazole, the resistant rates of the others increased year by year.