ukemia with high sensitivity and specificity. Platelet count is an essential para-clinical parameter determining the total response of the sensors. Follow up studies with a larger sample size are warranted to elucidate its clinical applicability.Polycyclic aromatic hydrocarbons are hazardous environmental pollutants that possess mutagenic and carcinogenic properties. Generally, the concentrations of PAHs in environmental water samples are very low, and it is challenging to detect such levels directly by the analytical instrumentation. Thus, the extraction of PAHs using suitable extraction methodology is required for sample cleanup and analyte enrichment. Dispersive solid-phase extraction has several advantages over conventional approaches for the extraction of PAHs from environmental water samples. In this article, we critically evaluate the role of different nano and micro sorbent materials employed in the extraction of PAHs. Carbon-based nanomaterials, metal-organic frameworks, polymeric nanocomposites, ionic-liquid based composites, and silica-based materials are explicitly covered. This review also provides insight on functional components of all types of sorbents and their way of interaction with PAHs. The factors affecting the dispersive (micro) solid phase extraction of PAHs such as the design of the sorbent, the ratio of functional material to magnetic core, sample volume, amount of sorbent, extraction and desorption times, desorption solvent and its volume, salt addition, and sample pH are critically appraised. Finally, a brief account on the accomplishments, limitations, and challenges associated with such methods is provided.Many forensic laboratories face growing demands for the processing of DNA evidence from sexual assault investigations. In these cases, evidence collected from the crime scene or from the victim in the form of a Sexual Assault and Evidence Collection Kit (SAECK) typically contains a mixture of cells from at least two donors. Isolation of DNA contributions to link a sample to an alleged offender requires precise chemical treatment of each sample with the goal of separating epithelial cells from non-sperm cells. Currently, the vast majority of laboratories employ differential chemical lysis methods that require lengthy incubations and several manual steps, preventing complete automation. Numerous alternative methods for the differential extraction (DE) of sexual assault evidence have been developed to provide a solution to the growing backlog of samples observed in the US and other countries. Here, we will discuss the predominant methodology for the DE of DNA from sexual assault samples and review alternative approaches from literature. We illustrate three criteria that provide a measure of success in performing these types of chemical separations and examine all methods based upon these expectations. We conclude by providing some general insight into the application of DE techniques in forensic laboratories and discuss the potential future directions of alternative technologies.Accurate measurement of naturally occurring radionuclides in blast furnace slag, a by-product of the steel industry, is required for compliance with building regulations where it is often used as an ingredient in cement. A matrix reference blast furnace slag material has been developed to support traceability in these measurements. Raw material provided by a commercial producer underwent stability and homogeneity testing, as well as characterisation of matrix constituents, to provide a final candidate reference material. The radionuclide content was then determined during a comparison exercise that included 23 laboratories from 14 countries. Participants determined the activity per unit mass for 226Ra, 232Th and 40K using a range of techniques. The consensus values obtained from the power-moderated mean of the reported participant results were used as indicative activity per unit mass values for the three radionuclides A0(226Ra) = 106.3 (34) Bq·kg-1, A0(232Th) = 130.0 (48) Bq·kg-1 and A0(40K) = 161 (11) Bq·kg-1 (where the number in parentheses is the numerical value of the combined standard uncertainty referred to the corresponding last digits of the quoted result). This exercise helps to address the current shortage of NORM industry reference materials, putting in place infrastructure for production of further reference materials.Accurate discrimination of common glycosaminoglycans (GAGs) before they are used in clinics is of great importance. Herein, a ratiometric sensor array Py-PP for discrimination of GAGs was constructed using three pyrene-porphyrin supramolecular complexes termed Py-PP1, Py-PP2 and Py-PP4. These complexes were readily synthesized by mixing pyrene-1-butyric acid (Py) and porphyrins PP1, PP2 and PP4 respectively. In the presence GAGs, the effective FRET from Py to porphyrin in the complex was influenced as a result of the competitive binding interactions between porphyrin and GAG. Controlled by the structural differences in the three porphyrins, complexes Py-PP1, Py-PP2 and Py-PP4 were determined to be cross-responsive towards tested GAGs including Hep, HA, Chs and DS. Distinctive fluorescence patterns were successfully generated for each GAG by the sensor array. The Py-PP sensor array was found to be powerful for discrimination of GAGs in both PBS and 5% serum media. Moreover, Py-PP was also successfully applied for reliable differentiation of Hep from other biological interferences and detection of trace GAG contaminants (0.1%, wt%) in Hep with 100% accuracy.At present, alpha fetoprotein (AFP) is mainly used as a serum marker of primary Hepatocellular carcinoma. A simple, enzyme-free sensing strategy is introduced for highly sensitive fluorescence detection of AFP. This detection strategy is based on aptamer recognition and mismatched catalytic hairpin assembly (MCHA). At first, Trigger is locked by aptamer before the introduction of AFP in this aptamer-MCHA system. The aptamer preferentially combines with AFP via powerful attraction in the presence of AFP. This results in the release of trigger and initiation of MCHA cycle, thus forming the H1 and H2 double chain complexes (denoted as H1@H2). Finally, H1@H2 and double chain structure containing fluorophore and its quenched group- BHQ1 (denoted as F@Q) initiated displacement reaction, which caused double chain separation and fluorescence recovery. This assay produces a wide detection range, which is from 0.1 ng mL-1 to 10 μg mL-1 and the limit of detection as 0.033 ng mL-1.  https://www.selleckchem.com/products/VX-770.html  The whole detection process was performed at 37 °C for 60 min.