This work aimed to investigate the feasibility of fabricating food-grade Pickering emulsions stabilized by soy protein isolate-chitosan (SPI-CS) nanoparticles. The SPI-CS nanoparticles were characterized by scanning electron microscopy and particle size analyzer. Pickering emulsion stabilized by SPI-CS nanoparticles were characterized by confocal laser scanning microscopy and optical microscopy. The results showed that the average size of SPI-CS nanoparticles was 148.52 ± 1.95 nm. The droplet sizes of Pickering emulsions at different nanoparticle concentrations and oil fractions were 11.80 ± 2.20-43.68 ± 4.12 μm. SPI-CS nanoparticles stabilized Pickering emulsions at different nanoparticle concentrations and oil fractions had low creaming index, and these Pickering emulsions were stable at 4 °C for 20 days. These SPI-CS Pickering emulsions were stable at broad ionic strengths (0-1000 mM) and temperature (4-60 °C). These findings may provide new insight into the development of food-grade Pickering emulsions suitable for the formulation of functional foods.The aim of this study was to develop chitosan (CS)/polycaprolactone (PCL) electrospun nanofibers with chlorogenic acid (CGA) loaded halloysite nanotubes (HNTs) as a carrier for CGA to provide a long-term sustained release. Results showed that the addition of CGA@HNTs at the concentrations of 2-6 wt% resulted in continuous nanofibers with an average diameter range of 159-166 nm, with improved thermal stability caused by the formation of hydrogen bonds. The incorporation of CGA@HNTs induced a hydrophilic surface and enhanced water vapor barrier property, but impaired the mechanical properties. However, the 2%, 4%, and 6% CGA@HNTs incorporated fibrous mats exhibited an initial release of 18.3 %, 26.2 %, 33.1 % within 15 h, and 34.7 %, 41.4 %, 51.3 % release after 240 h, respectively, indicating a long-term sustained release with enhanced antioxidant and antimicrobial activities. These results suggested the promising applications of the prepared CGA@HNTs/PCL/CS electrospun fibrous mats for active food packaging.Enzymatic glycosylation is an efficient way to increase the water solubility and the bioavailability of flavonoids. Levansucrases from Bacillus subtilis (Bs_SacB), Gluconacetobacter diazotrophicus (Gd_LsdA), Leuconostoc mesenteroides (Lm_LevS) and Zymomonas mobilis (Zm_LevU) were screened for puerarin (daidzein-8-C-glucoside) fructosylation. Gd_LsdA transferred the fructosyl unit of sucrose onto the glucosyl unit of the acceptor forming β-d-fructofuranosyl-(2→6)-puerarin (P1a), while Bs_SacB, Lm_LevS and Zm_LevU synthesized puerarin-4'-O-β-D-fructofuranoside (P1b) and traces of P1a. The Gd_LsdA product P1a was purified and assayed as precursor for the synthesis of puerarin polyfructosides (PPFs). Bs_SacB elongated P1a more competently forming a linear series of water-soluble PPFs reaching at least 21 fructosyl units, as characterized by HPLC-UV-MS, HPSEC and MALDI-TOF-MS. Simultaneous or sequential Gd_LsdA/Bs_SacB reactions yielded PPFs directly from puerarin with the acceptor conversion ranging 82-92 %. The bi-enzymatic cascade synthesis of PPFs in the same reactor avoided the isolation of the intermediate product P1a and it is appropriate for use at industrial scale.The agarose hydrogels (AG HyGels) were fabricated by a solvent casting method at various agarose concentrations, resulting in the 3D hydrogel networks via the physical crosslinking from the hydrogen bonding. The actuator performances were investigated at various agarose contents and electric field strengths. For the electromechanical properties, the AG HyGel_12.0 %v/v possessed the highest storage modulus (G') and storage modulus relative response (ΔG'/G'0) of 4.48 × 106 Pa and 1.07, respectively under applied electric field strength of 800 V/mm due to the electrostriction effect. In the electro-induced bending measurement, the highest deflection distance was obtained from the AG HyGel_2.0 %v/v due to its initial lower rigidity. Relative to other bio-based hydrogels, the present AG HyGels are first demonstrated here as electroactive materials showing comparable magnitudes in the electroactive responses, but with the simple fabrication method without toxic ingredients required. Thus, the present AG HyGels are potential material candidates for soft actuator applications.Branching poly (butylene succinate) (BPBS) nanocomposite foams incorporated with cellulose nanocrystals (CNCs) were prepared by supercritical CO2. https://www.selleckchem.com/products/FK-506-(Tacrolimus).html Surface modification of CNCs by acetylation was achieved through replacing hydrophilic hydroxyl groups with hydrophobic acetyl groups, which improved the dispersibility of CNCs significantly. The crystallite sizes of CNCs and acetylated CNCs were calculated by Scherrer's formula as 25 and 19 nm, respectively. The initial crystallization temperature of diverse poly (butylene succinate) (PBS) specimens, a crucial factor for regulating cell nucleation type, increased remarkably by 11.8 °C as well as their storage modulus increased by 2 orders of magnitudes, due to branching reaction and bio-filler addition. BPBS/CNCs foam possessed a high volume expansion ratio as 37.1 times and displayed an exceptional thermal conductivity as 0.021 W(m K)-1. This study provided a promising potential strategy to develop exceptional thermal-insulation polymer foams for composite structures, energy conservation and environment protection.This paper deals with the preparation of novel magnetic materials made from tetraaza macrocyclic Schiff base bacterial cellulose ligands with magnetite nanoparticles (Fe3O4NPs) through a multi-step procedure for antimicrobial and cytotoxic activities and chemotherapy in cancer treatment. First, the 2,3-dialdehyde bacterial cellulose (DABC) was chemically modified by ethylenediamine (EDA) and benzil (Bzl) in the presence of ferrous ions. Then, the magnetite nanoparticles (Fe3O4NPs) was produced inside the complex [Fe(DABC-EDA-Bzl)Cl2] through a co-precipitation method. In nanobiotechnology, the magnetic [Fe3O4NP-INS-(DABC-EDA-Bzl)] material was showed moderate antimicrobial and cytotoxic activities against different species and cells, respectively. In particular, the magnetic [Fe3O4NP-INS-(DABC-EDA-Bzl)] material have not any cytotoxic activity towards peripheral blood mononucleocyte (PBMC) cells. Anti-tumor studies demonstrated that the magnetic [Fe3O4NP-INS-(DABC-EDA-Bzl)] material effectively inhibits the growth of the CT26 tumor model in BALB/c mice compared with other resulting materials throughout the experimental period and can be effective drug delivery in nanomedicine.