from the chart.
  Rufinamide is a triazole derivative that is structurally dissimilar to other marketed antiepileptic drugs, has been assumed a marketing authorization, by the European Union and FDA, for use as a complementary therapy for seizures associated with Lennox-Gastaut syndrome.
 
  This work is concerned with development of two methods for determination of rufinamide (RUF) in presence of 1-[(2,6-difluorophenyl)methyl]-1H-1,2,3-triazole-4 carboxylic acid as its alkaline degradation product in dosage form.
 
  The first method was capable of determing RUF in the presence of its alkaline degradation product and in dosage form. Kromasil C8 column and mobile phase consisting of acetonitrile-water (5050, v/v) were used and UV detection at 210 nm. In the second method, first derivative ratio spectrophotometry, RUF was determined by measuring peak amplitude at 269.5 nm over 5-30 μg/mL.
 
  The linearity range of RUF was 10-90 μg/mL for HPLC method covering its therapeutic range with r2 = 0.9999. Forced degradation under alkaline conditions was carried out, the degradation product was isolated and its structure was confirmed. Both methods were validated in accordance to ICH guidelines. Statistical analysis revealed no significant difference between obtained results and reported ones.
 
  The present study is useful for therapeutic drug monitoring and routine analysis of RUF in quality control laboratories.
 
  Kinetics of the alkaline degradation of RUF was studied by following the concentration of the remaining drug until complete degradation was achieved.
 Kinetics of the alkaline degradation of RUF was studied by following the concentration of the remaining drug until complete degradation was achieved.
  Cefixime is a third-generation oral cephalosporin antibiotic widely used to treat bacterial infections. Typical methods for cefixime analysis use expensive instruments or sophisticated experimental procedures, and thus a sensitive and practical method is urgently needed for cefixime detection and analysis.
 
  To develop a sensitive and robust cefixime "switch-on" sensor based on carbon quantum dots (CQDs).
 
  In this study, black soya beans were used as an inexpensive carbon source for a "green" synthesis of fluorescent black soya bean (BS)-carbon quantum dots (CQDs). The fluorescence of these particles could be efficiently quenched by Ce(IV)due to the ground state recombination and electron transfer (ET) between Ce(IV)and BS-CQDs. In the presence of cefixime, the ET was interrupted and the fluorescent signal was recovered.
 
  This method showed high sensitivity and an impressively low detection limit of 169 nM.
 
  This low-cost, simple strategy for cefixime detection exhibits excellent stability, selectivity, and sensitivity. Moreover, it was successfully applied for the detection of cefixime in tablets and in a complex biological environment, confirming its great potential utility for drug analysis, biological process research, and clinical research.
 This low-cost, simple strategy for cefixime detection exhibits excellent stability, selectivity, and sensitivity. Moreover, it was successfully applied for the detection of cefixime in tablets and in a complex biological environment, confirming its great potential utility for drug analysis, biological process research, and clinical research.
  The GENE-UP®E. coli O157H7 2 (ECO 2) assay (Performance Tested MethodSM 121805) incorporates Fluorescence Resonance Energy Transfer hybridization probes into its proprietary PCR technology for the rapid detection of E.  https://www.selleckchem.com/products/Puromycin-2HCl.html  coli O157H7 in select foods.
 
  The purpose of this validation was to evaluate the method's interlaboratory performance and submit the result to AOAC INTERNATIONAL for adoption as First Action Official MethodSM for the detection of E. coli O157H7 in select foods.
 
  The GENE-UP® method was evaluated in a multi-laboratory study as part of the MicroVal validation process using unpaired test portions for one food matrix, raw milk cheese (Comté, 34% fat, 0.8% salt). The candidate method was compared to the ISO 166542001 reference method. Fourteen participants from 13 laboratories throughout the European Union participated. Three levels of contamination were evaluated a non-inoculated control level (0 colony-forming units (CFU)/test portion), a low contamination level (∼5 CFU/test portion), and a hi E. coli O157H7 assay provides industry with a rapid, accurate detection method for E. coli O157H7 in a broad range of foods.Some researchers, in their published articles in authentic scientific journals, plot calibration curves using bar charts instead of scatter plots using common software such as Excel. Bar charts can significantly affect the apparent linear range and sensitivity of the developed method, and using bar charts as calibration curves gives the wrong results. Therefore, this issue should be considered by researchers in developing analytical methods.
  Histamine fixed-immunoglobulin formulations, which consisted of 0.15 µg of histamine dihydrochloride and 12 mg of human immunoglobulin in a vial, are used for anti-allergic treatments, and controlling the amounts of histamine in the formulations is essential to avoid histamine intoxication.
 
  A high-performance liquid chromatography (HPLC) method for determination of histamine contents of the formulations was established and validated.
 
  Histamine extracted from the formulation was labeled with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate and was analyzed by gradient elution HPLC with UV detection at 260 nm.
 
  The method showed linearity in the range 0.8-2.4 µM (R > 0.999), accuracy (100.1-105.8% recovery), and precision (relative standard deviation ≤ 1.93%). The validated method was applied for five lots of the pharmaceutical, and their histamine contents were determined to be 0.149-0.155 µg/vial.
 
  These results indicated that the validated method is useful to control amounts of histamine in biopharmaceutical products.
 
  The HPLC method was developed for quantitative determination of histamine content of the histamine fixed-immunoglobulin formulations.
 The HPLC method was developed for quantitative determination of histamine content of the histamine fixed-immunoglobulin formulations.