https://www.selleckchem.com/products/nb-598.html Naphthalimide-benzothiazole conjugate (NBTZ) linked by cinnamonitrile was designed, synthesized, and fully characterized by NMR (1H, 13C, DEPT, HSQC) and high-resolution mass spectrometry. NBTZ exhibited unique turn-on fluorescence in the presence of CN- with relatively high selectivity compared to other anions such as SCN-, HSO4-, ClO4-, NO3-, Cl-, Br-, I-, and PO4-3 in tetrahydrofuran (THF). The detection limit for CN- was found to be 3.35 × 10-8 M in THF. The sensing mechanism was analyzed through 1H, 13C, DEPT, and mass spectroscopy. NBTZ also showed two-mode aggregation-induced emission (AIE) in THF-H2O mixtures. In a 3070 THF-H2O (v/v) mixture, the maximum AIE was observed at 430 nm (blue) because of the rotation of the CC bond between the naphthalimide ring and the phenyl ring was restricted. In 1090 THF-H2O (v/v), a new red-shifted AIE appeared at 490 nm (cyan), due to the extended π-conjugation induced by restriction of rotation of the CC bond between the benzothiazole and naphthalimide rings.In this work, a dual-functional Cu2+-based ensemble (2S·Cu2+) was well designed and characterized. Then, the successional and discriminating sensing for CN- over other competitive species (H2PO4- and biothiols) was achieved based on the disaggregation of 2S·Cu2+ ensemble and the deprotonation of imidazole NH of regenerated sensor S in aqueous solution, respectively. The visual sensing mechanism could be clearly demonstrated by 1H NMR, HRMS and energy changes between the HOMO-LUMO band gaps. Furthermore, the reversibility and reusability of S and 2S·Cu2+ upon alternating addition of CN-/H+ and CN-/Cu2+ were studied. Interestingly, the sequential sensing for biothiols (cysteine, glutathione and homocysteine) and CN- was also realized through spectroscopic methodology and test paper strips. This work may provide a feasible strategy to discriminate CN- over H2PO4- and biothiols with high selectivity and sensitivity through C