https://www.selleckchem.com/products/GDC-0449.html Concentrations of copper (Cu), iron (Fe), manganese (Mn), nickel (Ni) and zinc (Zn) in muscle and gills of four Benthic fish species (Brachirus orientalis, Argyrops spinifer, Platycephalus indicus and Sillago sihama) from Shif Island of Iran were analyzed using flame atomic absorption spectrometry. The heavy metal levels observed in tissues varied for Cu 2.15-820.98, Fe 24.46-533.26, Mn 1.80-53.17, Ni 1.86-65.54 and Zn 28.77-375.79 mg/kg. Significant differences were found among muscle and gill of the four fish species. Heavy metals concentration were significantly (p  less then  0.05) higher in the gill than in the muscles of all fish species studied. The metal levels in benthic fish species should be continuously monitored in polluted areas since benthic fish indicated a tendency to bioaccumulate pollutants in their tissues from polluted water. The indices of Estimated Daily Intake, Target Hazard Quotient and Hazard Index suggests no risk while carcinogenic risk for Ni showed unacceptable carcinogenic risk.Benzophenone-3 (BP-3) is an important ultraviolet (UV)-screening agent using in cosmetics, however, the associated environmental pollution and the toxicity to organisms, particularly aquatic organisms, cannot be neglected. In this study, the potential risks posed to zebrafish when exposed to environmental residual concentrations of BP-3 were evaluated. Zebrafish embryos (F0) were exposed to 0, 0.056, 2.3, and 38 μg/L BP-3 until 42 days' post-fertilization (dpf). The effects of BP-3 on the sex ratio and gene expression of F0 zebrafish were investigated. In the F1 embryos, cumulative hatching rate, body length, and heartbeats were observed. The result showed that F0 and F1 exposure to concentrations of 0.056 and 38 μg/L BP-3 elicited stronger toxicity at 96 hpf than single generation exposures. Overall, our results provide a new understanding on the effects of low BP-3 concentration chronic exposure on sex ratio a