OBJECTIVES The relationship between the molecular profiling of circulating tumor cells (CTCs) and clinical factors is a challenge. In this study, we performed molecular detection and characterization of CTCs in patients with head and neck squamous cell carcinoma (HNSCC). MATERIALS AND METHODS CTCs captured by microfilter were analyzed for the expression of multiple epithelial markers (EPCAM, MET, KRT19, and EGFR) by RT-qPCR. The CTCs-positive samples were further analyzed for the expression of 10 genes (PIK3CA, CCND1, SNAI1, VIM, CD44, NANOG, ALDH1A1, CD47, CD274, and PDCD1LG2). Finally, we analyzed whether the molecular profiling of CTCs was associated with clinical factors. RESULTS Twenty-eight (63.6%) of the 44 HNSCC patients were positive for at least one epithelial-related gene. CTC-positivity was significantly correlated with treatment resistance (p = 0.0363), locoregional recurrence (p = 0.0151), and a shorter progression-free survival (PFS) (p = 0.0107). Moreover, the expression of MET in CTCs was associated with a shorter PFS (p = 0.0426). Notably, patients with CD274-positive CTC showed prolonged PFS (p = 0.0346) and overall survival (p = 0.0378) compared to those with CD274-negative CTC. CONCLUSION Our results suggest that molecular profiling characterized by the gene expression of CTCs influences clinical factors in patients with HNSCC. Reasonable responsibility attribution and resource allocation in intragroup contexts benefit the evolution of group cooperation. Oxytocin (OT) has been shown to promote prosocial behavior; however, it remains unclear whether OT affects responsibility attribution and hypothetical resource allocation. In the present study, participants were intranasally administered OT or placebo (PLC) before a response task with a partner. The participant could win a certain amount of money depending on the group's performance, which was determined by the faster player. The contribution was manipulated to be similar in the first phase, while the participants could individually contribute more in the second phase. Our results show that both groups attributed more credit to the player who performed better in a trial. Moreover, reward magnitude only enhanced effort-based attribution in the OT group. Although both groups proposed to distribute money based on individual efforts, the PLC group increased their effort-based allocation when they contributed more, regardless of the fact that the money was eventually equally distributed. Our study demonstrates that OT modulates responsibility attribution and hypothetical resource allocation in different manners, suggesting that OT has different effects on a participant's perception of individual contribution and fairness when allocating a reward during social cooperation in a real effort task. Isolation of fiber concentrate enriched in β-glucan from barley flour via air currents assisted particle separation (ACAPS) generates an underutilized by-product stream, starch concentrate. Since barley starch concentrate (BSC) is depleted in soluble fibre, we examined the enzyme requirements for its hydrolysis and subsequent fermentation. Lower doses of a common raw starch hydrolyzing enzyme (STARGEN™ 002) effectively hydrolyzed BSC, achieving similar hydrolysis kinetics to the wheat benchmark.  https://www.selleckchem.com/products/ly333531.html  Hydrolysis of BSC did not require further enzyme supplementation, which is required for optimal wheat hydrolysis. This likely resulted from the smaller particle size of BSC relative to wheat feedstocks. Interestingly, simultaneous saccharification and fermentation of BSC using a 0.25X dose of STARGEN™ 002 alone enabled efficient ethanol production, though a requirement for phosphorus supplementation was identified. This study proposes a biorefining strategy that supports the generation of a value-added co-product, starch concentrate, while significantly reducing the enzyme requirements for bioethanol production. Crown All rights reserved.This study aimed to evaluate algal growth, lipid production, and nutrient removal in chicken farm flushing wastewater (CFFW). The excessive ammonia nitrogen (EAN) content in the CFFW wastewater represented a major factor limiting the algal growth. A strategy of mixing CFFW with municipal wastewater (MW) that contained less ammonia nitrogen was adopted. The results showed that the mixed wastewaters reduced ammonia nitrogen content, balanced nutrient profile, and promoted biomass production. The residual nutrients in mixed wastewaters were significantly reduced due to the algal absorption. Furthermore, alga grown on mixed wastewaters accumulated a higher level of total lipids and monounsaturated fatty acids that can be used for biodiesel production. The key issue of low biomass yield of algal grown on CFFW due to the inhibition of EAN was efficiently resolved by mitigating limiting factor to algal growth basing on mixing strategy, and accordingly the nutrients in the wastewater were significantly removed. The aim of this work was to study the ultrasonication-assisted Liquid Tri-phasic Flotation (LTF) System to obtain lipid and protein from microalgae Chlorella sorokiniana in a single step as a novel process. In the current study, biorefinery of Chlorella sorokiniana was performed using LTF system in a single step. The highest protein recovery of 97.43 ± 1.67% and lipid recovery of 69.50 ± 0.54% were obtained. The corresponding parameters were microalgae biomass loading of 0.5 w/v%, ammonium sulphate concentration of 40 w/v%, volume ratio of 11.5 (saltalcohol), ultrasonication pulse mode of 20 s ON/20 s OFF at 20% amplitude for 5 mins, flotation air flowrate of 100 mL/min. Additionally, recycling of alcohol phase to study the circular nature of proposed biorefinery was investigated. The proposed LTF system for extraction of proteins and lipid reduces the number of operation units required in this biorefinery approach. Due to its extensive application in agriculture as a germinating agent and growth promoter, indole acetic acid (IAA) is present in a variety of aquatic ecosystems. To explore the response of microbial aggregates to exogenous IAA in aquatic ecosystems, periphytic biofilm, a typical microbial aggregate, was exposed to IAA at different concentrations. Results reveal an unexpected concentration-dependent effect of IAA on periphytic biofilm. Concentrations of IAA less than 10 mg/L inhibit periphytic growth, but stimulate growth when the IAA concentration exceeds 50 mg/L. Periphytic biofilm adapts to different IAA concentrations by antioxidant enzyme activation, community structure optimization and carbon-metabolism pattern change, and promotes bioremediation of IAA contaminated water in the process. The removal rates of IAA reached up to 95%-100%. This study reveals the capacity of periphytic biofilm for IAA removal in practice.