https://www.selleckchem.com/ The inactivation mechanism of pathogenic microorganisms in water needs to be comprehensively explored in order to better guide the development of an effective and green disinfection method for drinking water safety. Here, metal-free modified g-C3N4 was prepared and used to inactivate two typical bacteria (namely, Gram-positive E. coli and Gram-negative S. aureus) in water under visible light from a comparative perspective. These two bacteria could be inactivated in the presence of modified g-C3N4 within 6 h of visible light, but their inactivation kinetics were quite different. E. coli were inactivated slowly in the early disinfection stage and rapidly in the later disinfection stage, whereas S. aureus were inactivated steadily during the entire disinfection process. Moreover, the impacts of important water parameters (pH, salt, temperature, and water matrix) on photocatalytic inactivation of E. coli and S. aureus were also distinct. In addition, scavenger experiments indicated that superoxide radicals played the most important role in E. coli inactivation, while both superoxide and hydroxyl radicals were important for S. aureus inactivation. Quantitative changes in fatty acids, potassium ions, proteins and DNA of the bacterial suspensions suggested that the higher resistance of E. coli in the early inactivation stage could be originated from the difference in the phospholipid repair system in cell membrane structures. This study can provide new insights into research and development of a safe and effective disinfection technology for drinking water.A tube-feeding model for administering microplastic (MP, Ø = 30 μm) spheres to fish larvae was employed to quantify the uptake of hydrophobic organic contaminants (HOCs) into the larval body through a single administration of MP. Polychlorinated biphenyl-153 (PCB-153) was used as a representative HOC that can be sorbed to MP in the sea. Atlantic herring (Clupea harengus) larvae (34-51 days po