This cross-sectional study with a cluster random sampling method was undertaken from November 2018 to May 2019 to estimate the prevalence and assess the potential risk factors of T. gondii seropositivity, and to assess the association of the T. gondii seropositivity with reproductive losses in small ruminants in Northwest Ethiopia. A total of 576 blood samples from 135 small ruminant flocks were collected and assayed for T. gondii IgG antibodies using the Toxo-latex slide agglutination test. A questionnaire survey was also conducted to gather information on the potential risk factors of T. gondii seropositivity and on the status of reproductive losses in the small ruminant flocks. The study demonstrated a high flock and animal level T. gondii seroprevalence of 96.3% (95% CI 91% - 98%) and 70.48% (95% CI 68% - 79%), respectively. Mixed effect logistic regression analysis of the potential risk factors showed that the odds of seropositive for T. gondii was significantly higher in sheep (OR = 1.7, 95%CI 1.19-2.46pathogen for the small ruminant producers. Therefore, it warrants control attention to reduce its economic and public health burden in the study areas. Further works on the economic impacts, bioassay, isolation, and genetic characterization of the pathogen are suggested.Tick-borne diseases (TBDs) caused by Theileria and Babesia spp. are common in tropical and subtropical regions. This study investigates the presence of Theileria and Babesia spp. in ruminants from a subtropical Mediterranean region (Sardinia, Italy), a hotspot for ticks infestations. A total of 141 blood samples from healthy and symptomatic ruminants (showing symptoms consistent with tick-borne disease) were screened using a polymerase chain reaction test based on the amplification of the 18 s rRNA fragment. A total of 19/50 sheep (38%), 34/43 bovine (79.1%), and 5/48 goats (10.4%) tested positive to Babesia/Theileria. Phylogenetic analysis assigned all sequences obtained from sheep to the T. ovis cluster, while bovine and goats sequence types grouped in the Theileria buffeli/sergenti/orientalis group. One sequence type, isolated from a symptomatic bovine, clustered with B. major. Information on presence and frequency of piroplasms in ruminants increase our knowledge about the circulation of these pathogens in Sardinian animals and add up to previous studies conducted in ticks in the same area. Results also highlight the importance of subtropical Mediterranean environments as hotspots for ruminants piroplasmosis with potential impact on Veterinary Health.Ancylostoma spp. are the most prevalent canine nematode parasites in Brazil. Despite their widespread parasitism in juvenile dogs, infections may occur regardless of host age. Although eosinophilia is a frequent finding in experimental infections, it is uncommon in naturally infected dogs. This study aimed to evaluate the prevalence of Ancylostoma spp. and the eosinophil blood counts (EBC) in naturally infected dogs, with or without comorbidities, admitted to the Veterinary Teaching Hospital of the São Paulo State University (UNESP), School of Veterinary Medicine and Animal Science, Botucatu campus, São Paulo state, Brazil, from 2009 to 2018. All retrospective data were gathered from veterinary medical records (VMR); diagnosis of Ancylostoma infection required the identification of eggs in fecal samples by the simple flotation test (SFT). Ancylostoma-infected animals were evaluated for other intestinal parasitic coinfections (IPC) by either the SFT or the centrifugal-flotation test.  https://www.selleckchem.com/products/CUDC-101.html  Dogs free of any gastro-inemiological findings should also be evaluated, since eosinophilia is less frequently observed with Ancylostoma spp. infections in the presence of comorbidities.An 8-year-old, spayed female Rottweiler dog, under immunosuppressant treatment for protein-losing enteropathy (PLE) and intestinal lymphangiectasia, was presented for anorexia, poor general conditions and episodes of diarrhea. A subcutaneous mass between the caudal abdominal mammary glands was found. A fine-needle aspiration cytology was performed and revealed the presence of inflammatory cells mixed with pear- or round-shaped microorganisms with cytomorphological features of flagellated protozoan trophozoites, belonging to Trichomonadida order. The final diagnosis of Tritrichomonas foetus infection was achieved with molecular analysis (Real-Time PCR) and sequencing. T. foetus has often been reported as a pathogenic agent in cattle and cats, and rarely reported in dogs, where its pathogenetic role is poorly understood. To the author's knowledge, this is the first case of T. foetus infection described in a location different from the gastrointestinal tract in a dog.The heartworm Dirofilaria immitis is the causative agent of cardiopulmonary dirofilariosis in dogs and cats and also infects humans. The current knowledge about this parasite in South America is scarce compared to North America and Europe. Nevertheless, this parasite has been reported in some regions of Colombia. The objective of this study was to determine the prevalence and risk factors associated with D. immitis infection in shelter dogs from Bucaramanga metropolitan area. We performed a cross-sectional study using blood samples from shelter dogs. Microfilariae testing of whole blood included a blood smear and modified Knott's test. Antigen testing of serum was performed using the Anigen Rapid HW Ag Test Kit 2.0 ®. A descriptive analysis and univariable logistic regression analysis were applied. A total of 207 whole blood samples, from 7 shelters dogs in the Bucaramanga metropolitan area, were utilized. The overall prevalence of D. immitis observed in shelter dogs in the Bucaramanga metropolitan area by blood smears and modified Knott's test was 6.3% (13/ 207) and 0.5% (1/207) by immunochromatography test kit. Regarding the prevalence by municipalities, there was a statistical association (P  .05). For Girón municipality there was a 7.1 times (OR = 7.1; CI95% 0.8-59.2; P  less then  .05) increased infection risk. This study provides current data and the first known report of the prevalence of D. immitis microfilaremia and antigenemia in shelter dogs in the Bucaramanga metropolitan area, and these results could be useful for designing new control measures for this infection. We observed that the prevalence of both microfilaremia and antigenemia was significantly lower in these sampled dogs compared to previous reports.