https://www.selleckchem.com/ the aim of this study was to analyze the chronobiology influence on the mechanical, kinematic, and physiological variables in a mountain bike (MTB) time trial. 16 mountain bike (MTB) male athletes volunteered to participate. Their characteristics were as follows body mass 70.2 ± 5.4 kg, stature 172.7 ± 4.0 cm, body fat 9.8 ± 3.5%, and VO 52.3 ± 3.9 mL/kg/min. Two 20 min MTB maximal protocols were applied, the first one in the morning and a second one in the afternoon period. No differences were found for all the variables studied, except for the pedaling cadence (stroke rate), which showed higher values during the morning protocol (85.06 ± 7.58 vs. 82.63 ± 7.41 rpm; = 0.044). Significant correlations between morning and afternoon physiological and mechanical variables were observed heart rate ( = 0.871); external mechanical power-maximum ( = 0.845), mean ( = 0.938), and relative ( = 0.933), as well as in the cadence-stroke rate ( = 0.825). our results reveal a similar impact and significant relationship between morning and afternoon impact concerning the majority of the physiological and mechanical variables, which indicates that the period of the day does not influence the external and internal impact associated with the MTB time trial maximal protocol. our results reveal a similar impact and significant relationship between morning and afternoon impact concerning the majority of the physiological and mechanical variables, which indicates that the period of the day does not influence the external and internal impact associated with the MTB time trial maximal protocol.This article introduces the spline approximation concept, in the context of system identification, aiming to obtain useful autoregressive models of reduced order. Models with a small number of poles are extremely useful in real time control applications, since the corresponding regulators are easier to design and implement. The main goal here is to compare the identificatio