https://www.selleckchem.com/products/smoothened-agonist-sag-hcl.html The purposes of this study were (1) to investigate the direct effect of an Er,CrYSGG laser on human apical papilla cell (APC) proliferation and mineralization and (2) to examine the effect of Er,CrYSGG laser, when applied to an ex vivo immature tooth model, on APC attachment. An Er,CrYSGG laser at various power outputs (0.1, 0.5, and 1 W) was used at different positions (2, 5, or 8 mm from the cells) to irradiate cultured APCs. APC proliferation and mineralization were assessed at various intervals. For the cell attachment evaluation, ex vivo tooth models containing dentin samples were irrigated with either EDTA or normal saline solution (NSS) and supplemented with laser activation. Fibronectin-positive-staining cells were counted and analyzed. The number of APCs was significantly greater when power outputs of 0.1 W and 0.5 W were used than when 1 W was used (P  less then  0.05). The close contact of laser application, at 2 and 5 mm, exerted a negative effect on cell proliferation at 24 and 48 h. The application at 8 mm did not show the deterioration effect. APC mineralization was reduced after laser irradiation, regardless of the power and the tip positioning, at 21 days. APC attachment in all laser-activated groups was significantly greater than in the groups without laser. The use of Er,CrYSGG laser significantly promoted APC attachment on the root canal dentin. Immediate implant placement with simultaneous contour augmentation such as guided bone regeneration (GBR) or connective tissue grafting (CTG) has been widely performed. However, few prospective studies have evaluated both peri-implant bone and soft tissue changes between the preoperative and postoperative periods. The purpose of this study is to quantify the horizontal dimensional changes of the facial bone and soft tissue following immediate implant placement with contour augmentation. Twenty patients who underwent immediate implant pla