Objective - to study the ability of N-oxide-2,6-dimethylpyridine to modify the cytogenetic effects in mouse bone marrow cells caused by an alkylating antitumor cytostatic cyclophosphamide.; The cytogenetic activity and mutagen-modifying effect of the plant growth regulator N-oxide-2,6-dimethylpyridine (Ivin) were studied by the method of accounting for chromosomal aberrations in the bone marrow cells of CD-1 mice (males) with a single joint exposure to cyclophosphamide. In the first variant of the research, Ivin was administered single orally in the form of an aqueous solution at doses of 710, 71, 7.1, 0.7, and 0.07 mg/kg bw, which corresponds to 1/2, 1/20, 1/200, 1/2000 and 1/20000 from LD50. In the second variant - Ivin was administered together with Cyclophosphamide (Ivin - in the same way as in the first research variant, cyclophosphamide was administered intraperitoneally at a dose of 40 mg/kg bw the same as the positive control group). Intact animals (negative control group) were orally administered purof Ivin, because of the stabilization of membranes and its antioxidant effect.The purpose of this scientific work was to investigate the development of puberty in females-offspring born to mothers of different age with fetoplacental insufficiency (FPI) and to evaluate efficacy of base and combined drug therapy during pregnancy. Negative influence of FPI on the puberty genesis of females-offspring born to mothers of different reproductive age is considered to be the results of the investigation. In particular, the increased anogenital distance, which is the sign of estrogen deficiency, has been observed in females-offspring born to reproductively young mothers with FPI. Females-offspring born to reproductively matured mothers with FPI have demonstrated more negative changes of reproductive system development. That is, body mass and anogenital distance increasing amid accelerated sexual development have been detected.  https://www.selleckchem.com/products/semaxanib-su5416.html  The increasing of testosterone level has caused inadequate ovaries stimulation which has led to steroid genesis disturbances. During histological investigation of ovarian structure of pubertal rats born to mothers of both groups of age, the decreasing of follicles density, the disturbance in follicles types ratio - early secondary follicles were prevailed, declining folliculogenesis reserve and increased number of atretical follicles have been observed. The introduction of pharmaceutical composition to pregnant rats of both groups of age amid fetoplacental insufficiency leads to stronger normalization of reproductive system development in females-offspring than using of drug of comparison.The aim of the study was elaboration of an approach for monitoring of cardiac output (CO) and systemic arterial pressure (SAP) using data obtained via measurements of the pulse waveform in peripheral arteries under steady-state and transitive conditions. CO and SAP were simultaneously recorded in common carotid and femoral arteries in narcotized (urethane, 1 mg/g) rats continuously and after infusions of sodium nitroprusside, adrenaline hydrochloride, dextran solution or acute experimental hemorrhage. Fourier analysis has been employed for estimating the generalized transfer functions (GTFs) and generalized vascular input impedance (GVI) along with individualized transfer functions (ITFs) for the states in the aftermath of infusions of vasoactive pharmacological agents, dextran solution or acute experimental hemorrhage. The results of pulse waveform analysis in the femoral artery were used for reconstruction of the pulse curves in carotid arteries and aortic blood flow. A comparison of directly measured and predicted pressure and flow values revealed the absence of significant differences under steady-state conditions. Short-term shifts of haemodynamics that follow various transitory influences on the cardiovascular system inevitably evoke changes in the mechanical properties of the blood vessels. Since both GTF and ITF express the mechanical properties of the vascular bed, their values also change under these conditions. This causes control of vascular stiffness and rigidity by estimation of the pulse wave velocities before and after administration of vasoactive agents and/or changes of the circulation blood volume.The aim of the work is to identify changes in the state of astrocytes and microglia with unilateral ligation of the carotid artery, sensitization with the cerebral antigen and their combination in the sensorimotor cortex in rats.; Studies were performed on 185 male Wistar white rats weighing 260-290 g. The brain was examined 1, 3, 10, 30 and 90 days after surgery and, respectively, 12 (1), 15 (3), 22 (10 ), 42 (30), 102 (90) days after sensitization (surgical intervention).; Immunohistochemical reactions were carried out in accordance with the manufacturer's protocols. Primary antibodies were used in the work S100 (Dako, Denmark); GFAP (Dako, Denmark), Iba-1. Densitometric measurements of S100 expression were performed using ImageJ 1.46 and counting the number of labeled GFAP and Iba-1 cells. Statistical processing was performed using t-student test.; In general, the observed reactions suggest that with discirculatory disturbances in the brain in rats, the detected changes in astrocytes and microglia tend to aused by it. When extrapolated to humans, it can be assumed that sensitization can make a significant contribution to the development of brain damage in conditions associated with hypoperfusion.The state of environmental hygiene of the urban dwelling environment plays a great role in the population health formation. The atmospheric air pollutants are among the principal factors affecting the immune system and provoking the development of a whole series of diseases. A wide distribution in a risk assessment system has gained a bio-monitoring methodology-based analysis for detecting toxic substances in human bio-substrates. For this purpose, a hair microelement analysis was conducted by an X-ray-fluorescence spectrometry technique to detect the content of lead in the bodies of children living in Tbilisi City under different conditions of environmental hygiene (according to the atmospheric air pollution degree). The average content of lead in the body of the children under study (according to hair analysis results) was found to be less than the maximum allowable concentration (MAC). At that, the content of lead in the children of the experimental group is 2,6-times more as compared with the control group, which is statistically reliable (Р less then 0,005).