001). In addition, a significant decrease in ALP concentrations in the group treated with the highest dose of black olive hydroalcoholic comparing with the OVX group was observed (P<0.001). The global, tibia, femur and spine BMD in the group treated with the highest dose of black olive hydroalcoholic and estrogen group were significantly increased compared to the OVX group (P<0.05).
 
  Black olive hydroalcoholic extract at the dose of 750 mg/kg, prevented bone loss and augmented bone mineral density and could be a possible candidate for the management of osteoporosis.
 Black olive hydroalcoholic extract at the dose of 750 mg/kg, prevented bone loss and augmented bone mineral density and could be a possible candidate for the management of osteoporosis.The molecular screening for laccase specific gene sequences in Trametes polyzona WRF03 (TpWRF03) using designed oligonucleotide primers analogous to the conserved sequences on the copper-binding regions of known laccases showed positive amplification with an amplicon size corresponding to 1500 bp. The purified TpWRF03 laccase (TpL) is a monomer with a molecular weight corresponding to 66 kDa. The enzyme had an optimal pH of 4.5 and temperature of 55 °C. TpL was most stable within pH of 5.5-6.5 and at a temperature range of 40-50 °C. Sodium azide, sodium cyanide and Fe2+ greatly inhibited the enzyme activity. TpL showed more than 50 % decolourisation efficiency on coomassie brilliant blue (72.35 %) and malachite green (57.84 %) but displayed low decolourisation efficiency towards Azure B (1.78 %) and methylene blue (0.38 %).  https://www.selleckchem.com/products/gw4869.html  The results showed that TpWRF03 produces high-yield of true laccase with robust properties for biotechnological applications.Cultivation of biomass crops for energy production is a promising land-use for farmland abandoned owing to radionuclide fallout. However, radionuclides in soil are easily taken up in the crop. To understand phase partitioning of radiocesium Cs (RCs) during anaerobic digestion (AD) of crops, semi-continuous AD experiments were carried out using two types of RCs-contaminated crops. Analysis of fractionated digestate effluent revealed that AD of the crops released RCs into the water phase (up to 82 %), and the efficiency of RCs solubilization depended on crop biodegradability. Adsorption treatment for removal of RCs from the water phase of the digestate indicated a water-zeolite partition coefficient of 0.287 L/g. The efficiency of removal from the water phase was 90 % at an adsorbent dose of 30 g/L.The development of cancer therapies has become difficult due to high metastasis, and lack of tissue selectivity, which in most cases affects normal cells. Demand for anticancer therapy is therefore increasing on daily basis. Gold nanoparticles (AuNPs) have many applications in biomedical field. Biological synthesis of AuNPs using aqueous extract of Crassocephalum rubens (AECR) was designed to investigate the in vitro anticancer potential. The synthesized AuNPs were characterized by UV-vis spectroscopy, high-resolution transmission electron microscopy, and Fourier transform infrared spectroscopy. The characterization results showed the formation of green AuNPs of wavelength 538 nm, and mostly spherical AuNPs with 20 ± 5 nm size. Significant anticancer activity of the AECR-AuNPs on MCF-7 and Caco-2 cells was noted at higher concentrations (125 and 250 μg/mL) during 24 and at all concentrations tested during 48 h. It can therefore be concluded that AECR leaves can mediate stable AuNPs with anticancer properties.The conventional analytical techniques used for the quantitative analysis of heavy metals in animal tissues are the atomic absorption spectroscopy (AAS) and the inductively-coupled plasma mass spectrometry (ICP-MS). These methods involve high cost, skilled personnel and long analysis times. Several studies have shown the applicability of electrochemical transducer coupled to disposable screen-printed electrodes (SPE) for measuring metals. The aim of the present study was to applicate and validate a simple and fast protocol for the simultaneous measurement of Cu and Pb concentrations in canine liver biopsy, coupling a simple digestion procedure with electrochemical stripping analysis. Square wave anodic stripping voltammetry (SWASV) coupled to disposable SPE was employed as fast and sensitive method for metals detection. Samples were digested with hydrogen peroxide/hydrochloric acid mixture for 1 h coupled to solid phase purification with carbon columns. Instrumental precision, digestion procedure accuracy andith AAS reference method.•Low cost, fast and sensitive method for diagnostic purposes.A new method for determining the interfacial tension of a magnetic fluid (MF) is proposed on the basis of deformation of a MF drop lying on a liquid substrate and subjected to a vertical uniform magnetic field. The results show that the drop elongates in the direction of the field with an increase of its intensity. As soon as the field strength reaches a certain value, the interface and the free surface of the drop become unstable, which causes the peaks of different height to form. It has been found that the ratio of the corresponding critical values of magnetic field intensity is determined by the ratio of surface tension at the interface to that on the free boundary of the drop. Surface and interfacial tension of liquids used in the experiment were measured with the help of tensiometer by the ring detachment method to verify the experimental data. The presented results on the ferrofluid interface tension measurements can be of interest for the specialists in the field of ferrohydrodynamics.•The magnetic field causes the drop to elongate till the peak instability.•The critical values of the field strength respond to the ferrofluid initial magnetic susceptibility.•The ratio of the critical magnetic field values is determined by the ratio of the interfacial tension.The ecological resource potential (ERP) method allows to calculate the amount of one material that can potentially be produced within Earth system boundaries, if no other anthropogenic activity would take place. It indicates the uppermost potential of one resource extracted, processed and disposed after use with a specific set of technologies and a defined probability of violating Earth system boundaries. This method is an adaption of the ecological resource availability (ERA) method, which calculates the amount of a resource that can be produced within an allocated share of the global boundaries, i.e. when considering all other anthropogenic activities. While more realistic, its allocation can be done in multiple ways and based on a variety of different objectives, which requires scenario modelling. The ERP method, in contrary, only requires information on environmental impacts from resource extraction, processing, and final disposal. The customization of the original ERA method comprises•Omitting all steps for allocating global boundaries to single resources or resource segments.