https://www.selleckchem.com/products/wst-8.html 4-3.6%). Similarly, the longitudinal pipelines showed a higher spatial reproducibility (1.1-7.8% of DICE improvement) in all hippocampal structures compared to long_T1s_FLAIR_crossT2. Moreover, long_T1s_FLAIR provided a small but significant RE improvement in comparison to long_T1s (p = 0.015), whereas no significant DICE differences were found. In addition, structures with volumes larger than 200 mm3 had better RE (1-2%) and DICE (0.7-0.95) than smaller structures. In summary, our study suggests that the most reproducible hippocampal subfield FreeSurfer segmentations are derived from a longitudinal pipeline using 3D-T1s and 3D-FLAIR. Adapting a longitudinal pipeline to include high-resolution 2D-T2 may lead to further improvements.A total of 60 healthy (the control group) and 30 anestrous Holstein cows with inactive ovaries (the case group) from three dairy farms around Tehran were randomly selected and diagnosed to determine the concentrations of glucose, insulin, and cortisol, and the rate of the insulin resistance disorder in them. Primarily, serum samples were obtained from all animals, and the concentrations of glucose, insulin, and cortisol were measured in a laboratory. Thereupon, the glucose tolerance test (GTT) was implemented. Before the implementation of the GTT, the median of serum glucose levels (62 mg/dl), insulin (7.16 μIU/ml), and cortisol (2.5 μg/dl) was estimated in healthy cows. The median of serum glucose levels (68.5 mg/dl, p = 0.00009) was higher, and serum insulin (6.1 μIU/ml, p = 0.025) and cortisol (1.1 μg/dl, p = 0.00029) levels were lesser in the anestrous cows when estimated simultaneously. After the implementation of GTT, the median of serum glucose concentrations in one (120 mg/dl), two (73.5 mg/dl), and three (63.5 mg/dl) hours after the glucose injection was estimated in healthy cows. The findings also showed that the median of serum glucose levels was lesser in one (100 mg/dl, p = 0.01