The aim is to develop a method of the acellular dermal matrix manufacturing from pig's skin dermis while preserving the native structure.
 
  Materials and methods Combination of physical and chemical effects on the dermis underlies in the process of an acellular dermal matrix manufacturing. Dermal collection of 1.0-1.3 mm thickness in pigs under 1 year of age from the back and partially from the lateral parts of the body was carried out.  https://www.selleckchem.com/products/liraglutide.html  The 0.3-0.4 mm thickness layer of skin was previously removed from the relevant areas with help of a dermatome, which was physically and chemically treated. The maximum acellularization of the dermal matrix was achieved step-by-step and included four stages of skin processing 1 - freeze-thaw process; 2 - glycerin dehydration; 3 - osmotic stress; 4 - cell residue removal by detergent.
 
  Results Histological analysis of the of the pig's skin dermis revealed that after freeze-thaw cycles the collagen scaffold of the dermal matrix maintains its structural organization that was oested protocol for decullalarization of the pig's skin dermis is effective in removing nuclear and cellular structures from dermis. Particular protocols can be modified by increasing the temperature difference or changing the number of freeze-thaw cycles.
  The aim To study the role and place of bone grafting in the formation of bone stump after amputation.
 
  Materials and methods 3 series of experiments were carried out on 44 rabbits with amputation of the thigh in the middle third and stump grafting using osteoplastic hermetic closure of the canal with a thin cortical plate (series I), closure of the canal with a spongy bone (series II), and loose closure of the canal with a cortical graft located at the entrance to the canal at an angle of 30° (ІІІ series). Observation period 1, 3, 6 months. Histological examination method with vascular filling with 10% mascara-gelatin mixture.
 
  Results In series I, in the majority of observations, a stump of a cylindrical shape with a bone locking plate of an osteon-beam structure and normalization of intraosseous microcirculation was formed. A slight displacement of the graft caused a violation of microcirculation. In series II, organotypic stumps were formed in all observations. In series III, incomplete closure of the bone marrow cavity led to sharp microcirculatory disorders and the course of the reparative process with pathological bone remodeling.
 
  Conclusions The parameters of the favorable course of the reparative process and the formation of the organotypic bone stump are the safety of its cylindrical shape, the presence of a compact bone structure, normalization of intraosseous microcirculation.
 Conclusions The parameters of the favorable course of the reparative process and the formation of the organotypic bone stump are the safety of its cylindrical shape, the presence of a compact bone structure, normalization of intraosseous microcirculation.
  The aim To obtain the first estimates of the current prevalence of healthcare-associated deep pelvic tissue infection and other infections of the female reproductive tract (vagina, ovaries, uterus) including chorioamnionitis, and antimicrobial resistance of causing pathogens in Ukraine.
 
  Materials and methods We performed a retrospective multicenter cohort study was based on surveillance data. The study population included 3,053 women's who underwent gynecological surgery or other procedures from 2017 to 2019 in 7 women hospitals in Ukraine. Definitions of healthcare-associated reproductive tract infection were used from the CDC/ NHSN.
 
  Results The prevalence of healthcare-associated deep pelvic tissue infection and other infections of the female reproductive tract was 26.3%. Incidence of infection was 13.3% Pelvic abscess or cellulitis, 14.6% Adnexa utery, 9.5% Salpingitis, 7.1% Oophoritis, 12.2% Parametritis, 4.6% Chorioamnionitis, and 38.8% Bacterial Vaginitis. The predominant pathogens were Escherichised by pathogens that are resistant to antibiotics.
  The aim To analyze the nutritional knowledge of women in the reproductive age about nutrients influencing the fetal nervous system's development.
 
  Materials and methods The study was conducted with a proprietary anonymous questionnaire using the CAWI (Computer Assisted Web Interviews) method among 263 women aged 18-51 years.
 
  Results In 35.4% of the surveyed women, the level of knowledge was insufficient, in 31.9% - sufficient, in 27% - good, and only in 5.7% - very good. The knowledge of the respondents was influenced by education (p < 0.001) and body mass index (BMI, p < 0.001). Women of reproductive age with higher education and/or lower BMI had a better understanding of nutrients influencing the fetal nervous system's development. Age, place of residence, family status, and professional status did not affect their knowledge.
 
  Conclusions The study showed that it is necessary to conduct nutritional education among women of reproductive age. Increasing the respondents' awareness of the importance of nutrients determining the fetus's proper development, both during the reproductive period and during pregnancy, is extremely important.
 Conclusions The study showed that it is necessary to conduct nutritional education among women of reproductive age. Increasing the respondents' awareness of the importance of nutrients determining the fetus's proper development, both during the reproductive period and during pregnancy, is extremely important.
  The aim The purpose of the present research was to study the results of coronary artery bypass graft (CABG) surgery depending on the degree of left ventricular ejection fraction (LVEF) reduction with the aim to identify additional echocardiographic predictors of the early postoperative period.
 
  Materials and methods Were fixed, the results of CABG in 97 patients operated on in the "Bikard" private clinic from March 2016 to December 2018 were the material of the research. All patients underwent CABG surgery under cardiopulmonary bypass and cardioplegia, and in the preoperative period underwent echocardiographic examination according to the standard technique on the Vivid 7 machine. Patients, in dependent of the LVEF, were divided into 3 groups group 1 35 people (LVEF < 40%), group 2 32 people ( 40% < LVEF < 50%), group 3 30 people (LVEF > 50%).
 
  Results Our studies showed that the most important echocardiographic predictors of a complicated development of the disease in the early postoperative period, in addition to LVEF of the heart, can be the size of the left ventricle and left atrial, the presence and severity of mitral regurgitation and diastolic dysfunction of the left ventricle of the heart.