4 to 12.7% (P0.1). In +MILK, the Nutrition Source affected piglet body composition (P less then 0.05) as piglets with low suckling frequency (LOW) had lower body fat and higher water content compared to piglets who had high suckling frequency (SUCKLE). Unexpectedly, drinking milk replacer in addition to suckling (MIXED) did not increase piglet body fat content. Relying mainly on milk replacer (CUP) caused body fat and water contents to be intermediate to piglets with high (SUCKLE and MIXED) and low suckling frequency (LOW). In conclusion, LS had a clear impact on piglet growth and body composition at weaning. In contrast, supplementation of milk and housing had only negligible impact on litter performance. Some individual piglets that had low frequency of sow milk intake benefitted from milk supplementation. Loose housing appeared to benefit piglet body fat at weaning but this was due to a greater piglet mortality.The current predation threat of domestic horses is generally low, and horses do not know predators' frightening cues. We studied whether horses still recognise predation threats. The aim of the study was to analyse the emotional response of purebred Arabian horses (Arabian) and Polish Konik horses (Konik) to an Arabian panther (Panthera pardus nimr) (panther) growl and a grey wolf (Canis lupus) (wolf) howl. Panther vocalisation was known to Arabian ancestors, whereas ancestors of Konik knew wolf vocalisation. The response to the howls of golden jackals (Canis aureus) (jackal), which did not prey on equids, was also studied comparatively. Two groups of 10 adult horses of each breed were subject to predator sounds of one predator daily for 5 min during a turn out on pasture. The test was performed for 18 days in total. The sound of each predator was interchangeably featured from one loudspeaker for 3 days followed by four loudspeakers simultaneously to imitate a group of predators for 3 days. The horses' emotioing of the sound meaning.  https://www.selleckchem.com/products/peg400.html  Their HRV response seems to be adequate for the threat signalised by the sound.Natural extracts are frequently adopted as a valuable alternative to antibiotics in intensive animal farming. Their diverse bioactive constituents such as phytosterols, glucosinolates, carotenoids and polyphenols have shown antioxidant, anti-inflammatory and antibacterial effects. Tannins are the largest class of polyphenol compounds of plant extracts, which can be classified into two hydrolysable or condensed subgroups. Poultry and swine nutrition are the most important sectors in which tannins have been used, firstly adopting tannin-rich feedstuffs and more recently, using tannin extracts from different plants. Several commercial products are available containing tannins extracted from the European chestnut tree (Castanea sativa Mill.) and the American quebracho (Schinopsis spp.). Tannins extracted from these plants have been applied on intensive swine farms due to their ability to improve animal performance and health. These positive and prominent effects are frequently associated with the antinutritional effects in reducing feed palatability, digestibility and protein utilization of feed. Some criticisms and contrasting results regarding pig performance and intestinal health have been reported. This paper provides an overview of the effects of chestnut and quebracho tannins on growth performance and intestinal health of pigs in order to clarify the appropriate dosage and response in the various physiological stages.Sperm vitrification has been recently developed, but fertility trials have not been performed yet in equine species. In this study, a new warming technique for vitrified donkey semen was developed and the uterine inflammatory response and fertility were compared to conventional freezing. In Experiment 1, sperm was vitrified in straws and warmed in 3 ml of extender or in a water bath at 37 °C/30 s; 43 °C/10 s; and 60 °C/5 s. Sperm motility, plasma and acrosome membranes and DNA integrity were compared between treatments. In Experiment 2, jennies were inseminated twice (500 × 106 sperm) in the uterine body either with vitrified or frozen semen (2 cycles/jenny). Pregnancy rates and the uterine inflammatory response (polymorphonuclear neutrophil concentration; PMN) were evaluated after artificial insemination (AI). No differences between warming in extender/water bath were found and 43 °C/10 s was better than lower temperatures in terms of total (53.8 ± 13.2%) and progressive sperm motility (41.4 ± 11.4%). No differences in PMN concentration (×103 PMN/ml) were found between vitrified (276.8 ± 171.6) or frozen (309.7 ± 250.7) semen after AI. However, PMN decreased faster (P  less then  0.05) using vitrified semen. Pregnancy rates were greater for vitrified (22%) than frozen semen (10%) but not statistically different. In conclusion, donkey sperm vitrified in straws could be directly warmed in a water bath at 43 °C/10 s, reducing the uterine inflammatory response obtained after AI and promoting positive pregnancy outcomes. These findings confirm the possibility to use vitrified semen as an alternative for AI in jennies.Preheating can increase the efficiency of commercial broiler breeding. A slow increase in temperature can counteract the negative effects of water condensation on the surface of the shell and reduces the thermal shock to the embryos. It is essential to design different preheating profiles because of the variances between breeds in terms of egg construction and the course of embryogenesis, along with the influence of the parental flocks' age on hatching rates. This study aimed to analyze six preheating profiles with different time and temperature patterns (A-F), which were immediately applied before the egg incubation began. These profiles were used for two broiler breeder strains - Hubbard Flex and Ross 308 - for the entire laying cycle divided into six age ranges 25-30, 31-36, 37-42, 43-48, 49-54, and 55-60wk of life. A total of 7839250 Hubbard Flex and 57167060 Ross 308 eggs were used in the study. The eggs were stored for 4-7d before the preheating profile was applied. Mortality during incubation, hatchability, and the quality of chicks were analyzed.