https://www.selleckchem.com/ 0% (P＜0.01) and 39.2% (P＜0.01), respectively; amitriptyline treatment significantly increased the number of Ki67+cells and DCX+ cells in the DG by 43.8% (P＜0.01) and 46.7% (P＜0.01), respectively, as compared with WKY rats. ② When compared with control group, WKY rats showed a significant decrease in sucrose preference (P＜0.01), less total horizontal distance (P＜0.01) and less time entered the center field (P＜0.01) in the OFT, the immobility time in the FST was increased significantly (P＜0.01). Amitriptyline treatment significantly improved the depression-like behaviors in WKY rats. CONCLUSION ① The proliferation and differentiation of hippocampal neural stem cells in adult WKY rats are significantly lower than those of Wistar rats, suggesting that the neurogenesis in adult WKY rats is impaired. ②Amelioration of impaired hippocampal neurogenesis can partially reverse the depression-like behaviors in adult WKY rats.OBJECTIVE Increasing activities in anterior cingulate cortex (ACC) can enhance the aversion reactions associated with noxious stimuli. It has been known that opioid receptors activation can trigger endogenous analgesic effect. This study tried to explore whether opioid receptors activation in the ACC region could reduce the aversion associated with noxious stimuli. METHODS The experimental rats were randomly divided into seven groups, Complete Freund's adjuvant (CFA) + normal saline (NS) group, normal saline (NS) + normal saline (NS) group, normal saline (NS) +DAMGO ((DAla2, NMe-Phe4, Gly-ol5)enkephinlin, μ-opioid receptor agonist) group, complete Freund's adjuvant (CFA)+ 0.01/0.04/0.2/1 μg/μl DAMGO group(n=6). The experimental period was three days. The basal value was measured on the first day. The second day, 1 μl was administered through the ACC area, and then 0.08 ml of complete Freund's adjuvant (CFA) was injected into the left hind paw of the rat. CPA response, paw withdrawal reflex latency (PWL) and electrical activity i