Titanium dioxide nanoparticles (TiO2-NPs) are widely applied as additives in foods due to their excellent whitening and brightening capability. Although the toxicity and antibacterial activity of TiO2-NPs have been extensively studied, their impact on the gut microbiota in vivo still remains unclear, especially in animals with gastrointestinal disorders. In the present study, healthy mice and TNBS-induced colitis mice were administered with TiO2-NPs (38.3 ± 9.3 nm) orally at a dose of 100 mg per kg bw daily for 10 days to study the impact of TiO2-NPs on the gut microbiota and colitis development. Moreover, the mechanism of TiO2-NPs on the gut microbiota was also discussed when the colitis mice were additionally administered with vitamin E to remove ROS. Changes in the microbiota community structure and gut-associated function prediction were analyzed through bioinformatics. The result showed that the oral administration of TiO2-NPs mitigated colitis symptoms by reducing the DAI and CMDI scores and TNF-α level. Furthermore, 16S rDNA sequencing analysis showed that the structure and function prediction of gut microbiota could be modified in healthy mice and colitis mice after exposure to TiO2-NPs, but the opposite physiological effect occurred since the dominant flora varied in these two groups. Moreover, vitamin E intervention did not change the effects of TiO2-NPs on the microbiota community structure and gut-associated function, which indicates that the mechanism of the biological effects of TiO2-NPs on the gut microbiota may not be associated with their ability to induce the generation of ROS. In summary, our work firstly found that TiO2-NPs could regulate the gut microbiota of colitis mice and participate in the mitigation of TNBS-induced acute colitis, and the capability of TiO2-NPs to induce the generation of ROS inducement did not contribute to this process.Flexible biosensors for monitoring systems have emerged as a promising portable diagnostics platform due to their potential for in situ point-of-care (POC) analytic devices. Assessment of biological analytes in sweat can provide essential information for human physiology. Conventional measurements rely on laboratory equipment. This work exploits an alternative approach for epidermal sweat sensing and detection through a wearable microfluidic thread/fabric-based analytical device (μTFAD). This μTFAD is a flexible and skin-mounted band that integrates hydrophilic dot-patterns with a hydrophobic surface via embroidering thread into fabric. After chromogenic reaction treatment, the thread-embroidered patterns serve as the detection zones for sweat transferred by the hydrophilic threads, enabling precise analysis of local sweat loss, pH and concentrations of chloride and glucose in sweat. Colorimetric reference markers embroidered surrounding the working dots provide accurate data readout either by apparent color ers in sweat, suggesting the great potential for development of feasible non-invasive biosensors, with a similar performance to conventional measurements.In recent years, hydrogel-based three-dimensional tumor models have become increasingly mainstream for cancer research. Hydrogels enable recapitulation of biochemical and biophysical cues in the tumor microenvironment (TME) for the culture of cancer and stromal cells. While there is increasing insight into how cancer-stromal interactions support tumor progression and drug resistance, much remains to be understood for the successful development of therapeutic targets that are capable of controlling tumors in patients. This review aims to first describe both acellular and cellular characteristics of the TME, focusing on cancer cell interactions with the extracellular matrix, fibroblasts, endothelial cells and immune cells. We will then discuss hydrogel systems that have been developed in the past four years to mimic these interactions in the TME and finally propose future directions in the field of in vitro tumor modeling.Aflatoxin B1 (AFB1) is one of the most carcinogenic chemicals. A novel fluorescence resonance energy transfer (FRET) sensor based on aptamer recognition technology is proposed for the sensitive detection of AFB1 in moldy peanuts using Ag nanocubes as energy acceptors and ZnS quantum dots (QDs) as energy donors.  https://www.selleckchem.com/products/XL184.html  Compared to the traditional FRET system based on an Au quencher, Ag nanocubes can not only quench the fluorescence of aptamer modified ZnS QDs, but are also inexpensive. In addition, compared with heavy metal QDs, ZnS QDs are environmentally friendly, have excellent photochemical properties, and are ideal energy donors. Without Ag nanocubes, the aptamer modified ZnS QDs emits blue fluorescence under an ultraviolet lamp. Because the emission spectrum of ZnS and the absorption spectrum of Ag nanocubes meet the requirements of FRET, the fluorescence quenching of ZnS QDs is realized. Nevertheless, with AFB1, the specific binding of aptamer and complementary chain makes the ZnS QDs break away from the Ag nanocubes, which leads to the fluorescence recovery of the ZnS QDs. Under the optimized detection conditions, the linear range of AFB1 was 5 pg mL-1 to 300 ng mL-1, and there was no obvious reaction with other similar mycotoxins. According to S/N = 3, the detection limit of AFB1 was 2.67 pg mL-1. The detection of AFB1 in peanut samples shows that the new FRET system can successfully be applied in the future to agricultural products.Cell-penetrating foldamers (CPFs) have recently shown promise as efficient and safe nucleic acid delivery systems. However, the application of CPFs to siRNA transport remains scarce. Here, we report helical CPFs tailored with specific end-groups (pyridylthio- or n-octyl-ureas) as effective molecular systems in combination with helper lipids to intracellularly deliver biologically-relevant siRNA.Asymmetric surface acoustic waves have been shown useful in separating particles and cells in many microfluidics designs, mostly notably sessile microdroplets. However, no one has successfully extracted target particles or cells for later use from such samples. We present a novel omnidirectional spiral surface acoustic wave (OSSAW) design that exploits a new cut of lithium niobate, 152 Y-rotated, to rapidly rotate a microliter sessile drop to ∼10 g, producing efficient multi-size particle separation. We further extract the separated particles for the first time, demonstrating the ability to target specific particles, for example, platelets from mouse blood for further integrated point-of-care diagnostics. Within ∼5 s of surface acoustic wave actuation, particles with diameter of 5 μm and 1 μm can be separated into two portions with a purity of 83% and 97%, respectively. Red blood cells and platelets within mouse blood are further demonstrated to be separated with a purity of 93% and 84%, respectively. These advancements potentially provide an effective platform for whole blood separation and point-of-care diagnostics without need for micro or nanoscale fluidic enclosures.