https://www.selleckchem.com/products/gsk126.html Aggregation process of riboflavin molecules in binary mixtures water - dioxane, water - DMSO, and ethanol - isobutanol, were investigated using spectroscopic methods and quantum-chemical calculation. It was shown that at a constant concentration of riboflavin and different ratios of binary mixtures, a deformation of the electronic absorption spectra with a hypochromic effect is observed. The observed changes are caused by the formation of a hydrogen bond and dipole-dipole interaction between riboflavin molecules, which is accompanied by a shift and resonance splitting of excited electronic levels.Direct laser cooling molecule is useful way to obtain the accurate molecular spectroscopy. However, most of the reported direct laser cooling schemes are only involved the molecules with a singlet or doublet ground state because the one with a triplet ground state is more complex, especially when the first-excited state is not suitable for the pseudo-closed loop transition. Using NH as the prototype of the simplest heteronuclear molecule with a triplet ground state, we focus on constructing the direct laser cooling scheme with a pseudo-closed loop triplet-triplet transition including intervening electronic states. The potential energy curves and transition dipole moments are calculated for the X3Σ-, a1Δ, b1Σ+, and A3Π states by using the multireference configuration interaction including spin-orbit coupling with the aug-cc-pV5Z basis sets. The rotational and vibrational energy levels of each electronic state are obtained by solving the Schrödinger equation of nuclear motion with the obtained potential energy curves. A two-color laser cooling scheme is established based on the 3Π1 → X3Σ- transition because the highly diagonal Franck-Condon factors make the transition suitable for constructing the pseudo-closed loop transition. The radiative lifetimes, the Doppler temperature, and the recoil temperature are calculated to access