Enzymatic results confirmed that the SOD nanoformulation reduced malondialdehyde expression and increased glutathione level in the ocular tissues, and thereby down-regulated oxidative stress and prevented RGC loss. Overall, this work offers a new therapeutic option for the treatment of retinal ischemic disorders by direct delivery of antioxidant proteins.The use of asparaginase (ASNase), a first line drug for lymphoma treatment, is impaired by short circulation and notoriously high immunogenicity. Although PEGylation can prolong the circulating half-life of ASNase, however, it also induces anti-PEG antibodies that lead to accelerated blood clearance (ABC) and hypersensitivity reactions. Here, we create an urchin-like polypeptide-ASNase conjugate P(CB-EG3Glu)-ASNase, in which the surface of ASNase is sufficiently shielded by an array of zwitterionic helical polypeptides through the labeling of the ε-amine of lysine. The conjugate is fully characterized with size exclusion chromatography, SDS-PAGE, dynamic light scattering, and circular dichroism. In vitro, P(CB-EG3Glu)-ASNase retains full activity based on the enzymatic assay using the Nessler's reagent and cell viability assay. In vivo, examination of the enzyme activity in serum indicates that P(CB-EG3Glu)-ASNase prolongs the circulating half-life of ASNase for ~20 fold. Moreover, P(CB-EG3Glu)-ASNase significantly inhibits tumor growth in a xenografted mouse model using human NKYS cells. Importantly, P(CB-EG3Glu)-ASNase elicits almost no antidrug or antipolymer antibodies without inducing ABC effect, which is in sharp contrast with a similarly produced PEG-ASNase conjugate that develops both antidrug/antipolymer antibodies and profound ABC phenomenon. Our results demonstrate that urchin-like conjugates are outstanding candidates for reducing immunogenicity of therapeutic proteins, and P(CB-EG3Glu)-ASNase holds great promises for the treatment of various lymphoma diseases.
  Advances in metabolomic tools have allowed us to gain a more comprehensive understanding of metabolic syndrome (MetS). The aim of this study was to evaluate the association between plasma metabolomic profiles and MetS.
 
  For this study, adults without diabetes, chronic kidney disease, stroke, heart disease, or cancer and with full metabolomics, biochemical, and dietetic data available, representing a subsample of the Health Survey of Sao Paulo study (ISA-Capital; N=130), were included. The joint interim statement consensus criteria were used for diagnosing MetS. Absolute quantification (µmol/L) of blood metabolites was achieved by targeted quantitative profiling of annotated metabolites by electrospray ionization tandem mass spectrometry in plasma samples. Mean differences in the compounds for MetS were evaluated by linear regression adjusted for confounding factors.
 
  Serine was inversely associated with MetS (β=-15.04; P=0.014). In glycerophospholipids with acyl-alkyl bonds, there was an inverse association with MetS, including phosphatidylcholine (PC) ae C425 (β=-0.15; P=0.040), PC ae C445 (β=-0.15; P=0.046), PC ae C404 (β=-0.21; P=0.014) and PC ae C444 (β=-0.04; P=0.032).
 
  Plasma metabolomic profiles were associated with MetS, especially the amino acid serine and some acyl-alkyl PCs.
 Plasma metabolomic profiles were associated with MetS, especially the amino acid serine and some acyl-alkyl PCs.
  Skeletal muscle mass with function decline indicated as sarcopenia, which may cause disability in elderly adults. Studies regarding fat composition in sarcopenia have gained attraction recently; however, different fat indexes have yielded different findings. It is necessary to explore the association between muscle mass, muscle function, and fat indexes among elderly adults.
 
  Community-dwelling elderly adults ages 65 and older who received annual health examination or outpatient services were enrolled. Hand grip strength and gait speed were measured.  https://www.selleckchem.com/products/iso-1.html  Muscle and fat mass were estimated by bioelectrical impedance analyzer. Presarcopenia was defined as loss of muscle mass only; sarcopenia was loss of muscle mass accompanied by low grip strength or/and slow gait speed. The relationships between sarcopenia parameters and different fat indexes among elderly adults were analyzed.
 
  There were 295 participants recruited. The presarcopenia group showed lower fat indexes compared to the sarcopenia group. Negative correlations existed between sarcopenia parameters (skeletal muscle mass index, grip strength, gait speed) and fat indexes (body-fat percentage, fat-to-muscle ratio). In the multiple hierarchical regression model, gait speed was negatively associated with body-fat percentage (β=-0.255, P=0.009) and fat-to-muscle ratio (β=-0.272, P=0.005) in the male group. In the female group, grip strength was inversely associated with body-fat percentage (β=-0.232, P=0.009) and fat-to-muscle ratio (β=-0.195, P=0.031).
 
  Individuals in the presarcopenia group had lower fat indexes than those in the sarcopenia group. Gait speed in men and hand grip strength in women-but not muscle mass for either- were negatively associated with body-fat percentage and fat-to-muscle ratio.
 Individuals in the presarcopenia group had lower fat indexes than those in the sarcopenia group. Gait speed in men and hand grip strength in women-but not muscle mass for either- were negatively associated with body-fat percentage and fat-to-muscle ratio.The preservation of cultural heritage is very important. Ionizing radiation is widely used for this purpose and use of gamma irradiation has become common practice in cultural heritage preservation. The number of available electron accelerators is two orders of magnitude greater than for gamma facilities, and many countries do not have gamma facilities. In this work we demonstrate the possibility of using an electron accelerator and X-rays generated from accelerated electrons for radiation treatment of wooden cultural heritage. The GEANT4 toolkit was used for the simulation of the passage of particles through matter. The accuracy of radiation treatment numerical simulation in comparison with experiment was in range of 1.5-11.8%.