https://www.selleckchem.com/JAK.html Every human smile is unique and special, reflecting the different shapes, textures, and colors of the teeth as well as their combinations. This uniqueness conveys a direct and specific message when it comes to creating a new smile design. Dentists need to consider the 'unity of the whole' concept in this task, which involves bringing together biology, structure, function, and esthetics to create the fifth dimension of the smile. This approach is quite different from the traditional one. A smile reflects the personality and emotional state of the patient, and the translation of this into a new smile design occurs in various ways. This can be performed either through analog methods or digitally; however, the use of visual language will assist the dentist and dental technician in the creation of a personalized smile design. Nowadays, with the significant digital advances that have occurred in dentistry, there are systems on the market that help the dental team to create a personalized new smile design for each specific patient. The Rebel system is one example of such a system. It is actually a virtual laboratory that creates individualized smile designs in three dimensions. The system, with its very sophisticated artificial intelligence (AI)-based software, immediately creates a digital wax-up (STL file). It is therefore simple to use for dentists and dental technicians at all skill levels because the direct mock-up and wax-up stages are eliminated. Embryos are usually produced in culture systems with an oil overlay, which conveys protection against the evaporation of water and microbial contamination. The oil can also release toxic substances and absorb essential components, such as hormones, which adversely affect the quality of the oocytes and the development of embryos in vitro. The aim of this study was to validate an oil-free bovine in vitro production (IVP) system. Cumulus-oocyte complexes collected from abattoir-derived ova