This approach is applicable during the nanofabrication of various high-resolution devices with complex geometrical nanopatterns.Hepatocellular carcinoma (HCC), as a well-vascularized tumor, has attracted increasing attention in antiangiogenic therapies. Notably, emerging studies reveal that the long-term administration of antiangiogenic drugs induces hypoxia in tumors. Pericytes, which play a vital role in vascular stabilization and maturation, have been documented to be associated with antiangiogenic drug-induced tumor hypoxia. However, the role of antiangiogenic agents in regulating pericyte behavior still remains elusive. In this study, by using immunostaining analysis, we first demonstrated that tumors obtained from HCC patients were highly angiogenic, in which vessels were irregularly covered by pericytes. Therefore, we established a new 3D model of tumor-driven angiogenesis by culturing endothelial cells, pericytes, cancer stem cells (CSCs) and mesenchymal stem cells (MSCs) with microcarriers in order to investigate the effects and mechanisms exerted by antiangiogenic agents on pericyte recruitment during tumor angiogenesis. Interestingly, microcarriers, as supporting matrices, enhanced the interactions between tumor cells and the extracellular matrix (ECM), promoted malignancy of tumor cells and increased tumor angiogenesis within the 3D model, as determined by qRT-PCR and immunostaining. More importantly, we showed that zoledronic acid (ZA) reversed the inhibited pericyte recruitment, which was induced by sorafenib (Sora) treatment, through fostering the expression and activation of ErbB1/ErbB2 and PDGFR-β in pericytes, in both an in vitro 3D model and an in vivo xenograft HCC mouse model. Hence, our model provides a more pathophysiologically relevant platform for the assessment of therapeutic effects of antiangiogenic compounds and identification of novel pharmacological targets, which might efficiently improve the benefits of antiangiogenic treatment for HCC patients.Comprehensive multiphase NMR combines the ability to study and differentiate all phases (solids, gels, and liquids) using a single NMR probe. The general goal of CMP-NMR is to study intact environmental and biological samples to better understand conformation, organization, association, and transfer between and across phases/interfaces that may be lost with conventional sample preparation such as drying or solubilization. To date, all CMP-NMR studies have used 4 mm probes and rotors. Here, a larger 7 mm probehead is introduced which provides ∼3 times the volume and ∼2.4 times the signal over a 4 mm version.  https://www.selleckchem.com/products/jzl184.html  This offers two main advantages (1) the additional biomass reduces experiment time, making 13C detection at natural abundance more feasible; (2) it allows the analysis of larger samples that cannot fit within a 4 mm rotor. Chicken heart tissue and Hyalella azteca (freshwater shrimp) are used to demonstrate that phase-based spectral editing works with 7 mm rotors and that the additional biomass from the larger volumes allows detection with 13C at natural abundance. Additionally, a whole pomegranate seed berry (aril) and an intact softgel capsule of hydroxyzine hydrochloride are used to demonstrate the analysis of samples too large to fit inside a conventional 4 mm CMP probe. The 7 mm version introduced here extends the range of applications and sample types that can be studied and is recommended when 4 mm CMP probes cannot provide adequate signal-to-noise (S/N), or intact samples are simply too big for 4 mm rotors.A colorimetric sensor array based on complexometric indicators is proposed for pattern recognition of lanthanide ions. The complexometric indicators have abundant functional groups and can act as a platform for chromogenic reaction with various metal ions, including lanthanide ions. The subtle difference of the lanthanide ions' structure results in the difference of absorbance response between lanthanide ions and two chromogenic indicators (Alizarin Red and Erichrome Black T) in Tris-HCl buffer with two different pHs (i.e., pH 7.4 and pH 8.5, colorimetric sensor array). Fourteen lanthanide ions were distinguished well with the newly designed colorimetric sensor array. The sensor array has the potential to distinguish between different concentrations of lanthanide ions and their mixtures. Moreover, the results in actual samples indicate the future practical applications of this sensor array in environmental analysis.Hollow porous graphitic carbon nitride (porous CN) was synthesized via a simple tactic method, and the resulting porous CN showed an effectively modified surface area, crystal structure and enhanced photocatalytic performance. Optical and electrochemical characterization results demonstrated an increase in the charge transfer rate and a decrease in recombination tendency.Correction for 'Synchrotron macro ATR-FTIR microspectroscopy for high-resolution chemical mapping of single cells' by Jitraporn Vongsvivut et al., Analyst, 2019, 144, 3226-3238, DOI 10.1039/C8AN01543K.Coordination compounds with redox-active ligands are currently intensively studied. Within this research theme, redox-active guanidines have been established as a new, eminent class of redox-active ligands. In this work the variation of metal-guanidine bonding in dinuclear transition metal complexes with bridging redox-active tetrakisguanidine ligands is analysed. A series of dinuclear complexes with different metals (Mn, Fe, Co, Ni, Cu and Zn) is synthesized, using either newly prepared redox-active tetrakisguanidino-dioxine or previously reported tetrakisguanidino-benzene ligands. The discussion of the bond properties in this work is predominantly based on the trends of structural parameters, derived from determination of single-crystal structures by X-ray diffraction and quantum chemical calculations. In addition, the trends in the redox potentials and magnetometric (SQUID) measurements on some of the complexes are included. Due to their combined σ- and π-electron donor capability, redox-active guanidine ligands are weak-field ligands; the σ- and π-bonding contributions vary with the metal. The results highlight the peculiarity of copper-guanidine bonding with a high π-bond contribution to metal-guanidine bonding, enabled by structural distortion of the coordination mode from tetrahedral in the direction of square-planar, short copper-guanidine bonds and minor displacement of the copper atoms from the ligand aromatic plane.