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https://www.selleckchem.com/products/img-7289.html Objective To investigate the effect of perfluorooctane sulfonate (PFOS) on inflammatory factors in human placental trophoblast (HTR-8/Svneo) cells. Methods HTR-8/Svneo cells were exposed to different concentrations of PFOS (0, 0.01, 0.1, 1.0 mg/L) for 24 h, and the cell survival rates were measured by CCK8. Secretion levels of interleukin-6 (IL-6) , tumor necrosis factor-alpha (TNF-α) and interleukin-10 (IL-10) were detected by ELISA. The mRNA expressions of IL-6, TNF-α and IL-10 were detected by fluorescence quantitative PCR. One-way ANOVA was used to analyse the expressions of inflammatory factors. Results Compared with the control group, the survival rates of 0.1 and 1.0 mg/L PFOS groups were significantly decreased (P less then 0.05) . Compared with the control group, the secretion levels of IL-6 were decreased in the 0.01, 0.1 and 1.0 mg/L PFOS groups (P less then 0.05) , the concentrations of TNF-α were increased in the 0.01 and 1.0 mg/L PFOS groups (P less then 0.05) , and the concentrations of IL-10 were increased in the 0.1 and 1.0 mg/L PFOS groups (P less then 0.05) . Compared with the control group, the expressions of IL-6 mRNA were increased in the 0.1 and 1.0 mg/L PFOS groups (P less then 0.05) , and the expressions of IL-10 mRNA were decreased in the 0.01 mg/L, 0.1 mg/L and 1.0 mg/L PFOS groups (P less then 0.05) . Conclusion PFOS can induce changes in the secretion levels of inflammatory cytokines in HTR-8/Svneo cells, resulting in decreased activity of placental trophoblast cells and abnomal placental function.Objective To investigate the clinical value of the first multicolor fluorescence in situ hybridization (FISH) assay on multiple genes, and combined with 9p21 and 8q24 evaluation in the differential diagnosis of melanoma. Methods Fifty-six melanomas and 36 benign melanocytic nevi diagnosed in Fudan University Shanghai Cancer Center from 2017 to 2019 were included. Each specimen was examined by f
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