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https://www.selleckchem.com/products/tat-beclin-1-tat-becn1.html Wnt signaling dysfunction and gut dysbiosis may lead to liver fibrosis, yet the underlying mechanisms are not well elucidated. This study demonstrated the role of RSPO4, a Wnt signaling agonist, in liver fibrogenesis and its impact on the gut microbiome. RSPO4 gene in CCl4-induced fibrotic-liver rats was knockout by Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) system, with healthy rats served as the control. Tissue samples and hepatic stellate cells (HSCs) isolated from rats were examined for curative effect of RSPO4-CRISPR treatment. Fecal sample were collected and analyzed with 16 S rRNA sequencing. We found RSPO4-CRISPR relieved liver fibrosis in rats and reversed HSC activation. Further, results showed RSPO4-CRISPR tended to restore the microflora composition. Significance species between groups were identified. Bacteroides and Escherichia-Shigella were the key microbes in the model and negative group, whereas Lactobacillus, Romboutsia, and Lachnospiraceae NK4A136 group were abundant in the control. Notably, Bacteroidales S24-7 group and Ruminococcaceae UCG-005 were the significantly enriched in CRISPR group. We show that the microbiome of rats treated with RSPO4-CRISPR presents a trend towards the restoration of the original condition. Our findings pave a new way to evaluate the curative effect of liver fibrosis treatment.An autonomous underwater vehicle (AUV) was deployed in Lake Biwa from 2000 to 2012. In December 2009, ebullition of turbid water was first found in the deepest area (> 90 m) of the North Basin. Follow-up investigations in April and December 2010 and January 2012 confirmed the existence of benthic vents similar to the vents observed in other deep lakes. Importantly, vent numbers per unit travel distance in Lake Biwa dramatically increased from only two vents (0.37 vents km-1) in December 2009 to 54 vents (5.28 vents km-1) in January 2012, which could be relat

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